| Title: | Biological and chemical strategies for exploring inter- and intra-kingdom communication mediated via bacterial volatile signals |
| Author(s): | Farag MA; Song GC; Park YS; Audrain B; Lee S; Ghigo JM; Kloepper JW; Ryu CM; |
| Address: | "Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt. Molecular Phytobacteriology Laboratory, Infectious Disease Research Center, Korea Research Institute of Bioscience &Biotechnology (KRIBB), Daejeon, South Korea. Genetics of Biofilms Unit, Department of Microbiology, Institut Pasteur, Paris, France. Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama, USA. Department of Biosystems and Bioengineering, University of Science and Technology, Daejeon, South Korea" |
| ISSN/ISBN: | 1750-2799 (Electronic) 1750-2799 (Linking) |
| Abstract: | "Airborne chemical signals emitted by bacteria influence the behavior of other bacteria and plants. We present an overview of in vitro methods for evaluating bacterial and plant responses to bacterial volatile compounds (BVCs). Three types of equipment have been used to physically separate the bacterial test strains from either other bacterial strains or plants (in our laboratory we use either Arabidopsis or tobacco plant seedlings): a Petri dish containing two compartments (BI Petri dish); two Petri dishes connected with tubing; and a microtiter-based assay. The optimized procedure for the BI Petri dish system is described in this protocol and can be widely used for elucidation of potential function in interactions between diverse microbes and those plant and chemical volatiles emitted by bacteria that are most likely to mediate bacterial or plant responses to BVCs. We also describe a procedure for metabolome-based BVC profiling via dynamic (i.e., continuous airflow) or static headspace sampling using solid-phase microextraction (SPME). Using both these procedures, bacteria-bacteria communications and bacteria-plant interactions mediated by BVCs can be rapidly investigated (within 1-4 weeks)" |
| Keywords: | Arabidopsis/*microbiology Bacteria/*metabolism *Host-Pathogen Interactions *Microbial Interactions Tobacco/*microbiology Volatile Organic Compounds/*metabolism; |
| Notes: | "MedlineFarag, Mohamed A Song, Geun Cheol Park, Yong-Soon Audrain, Bianca Lee, Soohyun Ghigo, Jean-Marc Kloepper, Joseph W Ryu, Choong-Min eng England 2017/06/16 Nat Protoc. 2017 Jul; 12(7):1359-1377. doi: 10.1038/nprot.2017.023. Epub 2017 Jun 15" |
