| Title: | "Chromatographic analysis, antimicrobial and insecticidal activities of the essential oil of Phlomis floccosa D. Don" |
| Author(s): | El Mokni R; Majdoub S; Chaieb I; Jlassi I; Joshi RK; Hammami S; |
| Address: | "Laboratory of Botany and Plant Ecology, Faculty of Sciences of Bizerta, University of Carthage, Bizerta, Tunisia. Silvo-Pastoral Resources Laboratory, Silvo-Pastoral Institute of Tabarka, University of Jendouba, Tunisia. Department of Botany and Plant Biology, Faculty of Pharmacy of Monastir BP 207, University of Monastir, Tunisia. Research Unit 13ES63, Applied Chemistry and Environment, Monastir University, Faculty of Sciences of Monastir, University of Monastir, Tunisia. Regional Centre of Research on Horticulture and Organic Agriculture, University of Sousse, Sousse, Tunisia. Transmissible Diseases and biologically active substances LR99ES27, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia. Department of Phytochemistry, ICMR-National Institute of Traditional Medicine, Karnataka, India" |
| ISSN/ISBN: | 1099-0801 (Electronic) 0269-3879 (Linking) |
| Abstract: | "The present study was conducted to investigate for the first time the chemical composition and insecticidal and antimicrobial properties of Phlomis floccosa D. Don. essential oil isolated by hydro-distillation of its aerial parts. Qualitative and quantitative analyses of the essential oil on the basis of gas chromatography and mass spectrometry (GC/FID and GC/MS) revealed the presence of 59 organic volatiles representing 86.9% of the total constituents. The major compounds were germacrene-D (19.7%) followed by beta-caryophyllene (15.5%), caryophyllene oxide (8.3%), hexadecenoic acid (7.9%) and carvacrol (6.1%). The broth microdilution method was conducted to test the antimicrobial activity of the essential oil against five pathogens: Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, Bacillus subtilis and the yeast Candida albicans. The oil of P. floccosa D. Don. shows promising activity against the Gram-positive bacteria S. aureus and B. subtilis and the yeast C. albicans (minimum inhibitory concentration = 625 mug.mL(-1) ). The effectiveness of the essential oil was assessed on both Tribolium confusum and Sitophilus zeamais pest insects and it was more pronounced against T. confusum, showing a moderate mortality of 47.5%" |
| Keywords: | "Animals Anti-Infective Agents/analysis/chemistry/pharmacology Bacteria/drug effects Coleoptera/drug effects Fungi/drug effects Gas Chromatography-Mass Spectrometry *Insecticides/analysis/chemistry/pharmacology Microbial Sensitivity Tests *Oils, Volatile/a;" |
| Notes: | "MedlineEl Mokni, Ridha Majdoub, Siwar Chaieb, Ikbal Jlassi, Ibrahim Joshi, Rajesh K Hammami, Saoussen eng England 2019/05/23 Biomed Chromatogr. 2019 Oct; 33(10):e4603. doi: 10.1002/bmc.4603. Epub 2019 Jun 24" |
