| Title: | Extraction and characterization of bioactive secondary metabolites from lactic acid bacteria and evaluating their antifungal and antiaflatoxigenic activity |
| Author(s): | Abdel-Nasser A; Hathout AS; Badr AN; Barakat OS; Fathy HM; |
| Address: | "Food Toxicology and Contaminants Department, National Research Centre, Egypt. Agricultural Microbiology Department, Faculty of Agriculture, Cairo University, Egypt" |
| DOI: | 10.1016/j.btre.2023.e00799 |
| ISSN/ISBN: | 2215-017X (Print) 2215-017X (Electronic) 2215-017X (Linking) |
| Abstract: | "Aflatoxins are toxic carcinogens and mutagens formed by some moulds, specifically Aspergillus spp. Therefore, this study aimed to extract and identify bioactive secondary metabolites from Lactobacillus species, to evaluate their efficacy in reducing fungal growth and aflatoxin production and to investigate their toxicity. The bioactive secondary metabolites of Lactobacillus species showed variable degrees of antifungal activity, whereas L. rhamnosus ethyl acetate extract No. 5 exhibited the highest antifungal activity and, thus, was selected for further identification studies. Data revealed that L. rhamnosus ethyl acetate extract No. 5 produced various organic acids, volatile organic compounds and polyphenols, displayed antifungal activity against A. flavus, and triggered morphological changes in fungal conidiophores and conidiospores. L. rhamnosus ethyl acetate extract No. 5 at a 9 mg/mL concentration reduced AFB(1) production by 99.98%. When the effect of L. rhamnosus ethyl acetate extract No. 5 on brine shrimp mortality was studied, the extract attained a 100% mortality at a concentration of 400 microg/mL, with an IC(50) of 230 microg/mL. Meanwhile, a mouse bioassay was performed to assess the toxicity of L. rhamnosus ethyl acetate extract No. 5, whereas there were no harmful effects or symptoms in mice injected with L. rhamnosus ethyl acetate extract at concentrations of 1, 3, 5, 7, and 9 mg/kg body weight" |
| Keywords: | Aflatoxins Antifungal activity Bioactive secondary metabolites Brine shrimp bioassay Lactic acid bacteria Mouse bioassay; |
| Notes: | "PubMed-not-MEDLINEAbdel-Nasser, Aya Hathout, Amal S Badr, Ahmed N Barakat, Olfat S Fathy, Hayam M eng Netherlands 2023/05/19 Biotechnol Rep (Amst). 2023 May 3; 38:e00799. doi: 10.1016/j.btre.2023.e00799. eCollection 2023 Jun" |
