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Mutat Res

Title:		"Genotoxic and mutagenic activity of environmental air samples in Flanders, Belgium"
Author(s):		Du Four VA; Van Larebeke N; Janssen CR;
Address:		"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Ghent, Belgium. vincent.dufour@ugent.be"
Journal Title:		Mutat Res
Year:		2004
Volume:		558
Issue:		1-Feb
Page Number:		155 - 167
DOI:		10.1016/j.mrgentox.2003.12.002
ISSN/ISBN:		0027-5107 (Print) 0027-5107 (Linking)
Abstract:		"Atmospheric pollution is assumed to play a role in the incidence of respiratory diseases and cancers. Airborne particles are able to penetrate deep into the lung and are composed of complex chemical mixtures, including mutagens and carcinogens such as polycyclic aromatic compounds (PACs). The present study reports mutagenic and genotoxic activities associated with ambient air collected near a busy street in Borgerhout, at an industrial site in Hoboken and in Peer, a rural community 70 km east of Antwerp in Flanders, Belgium. Airborne particulates (PM10) and semi-volatile organic compounds were sampled during winter and summer. Samples were collected with a high-volume sampler using quartz filters (QF) and polyurethane foam (PUF) cartridges. The mutagenic and genotoxic activity of the organic extracts was determined using the Salmonella test/standard plate-incorporation assay and the Vitotox assay. Concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) in the extracts were determined by reversed-phase high-performance liquid chromatography (HPLC). The mutagenicity assay, using Salmonella typhimurium strain TA98, demonstrated direct mutagenicity of up to 58 revertants/m3 for the QF extracts and low or no mutagenic activity in the PUF extracts. Metabolic activation of the samples resulted in high indirect mutagenicity for both QF and PUF extracts: up to 96 revertants/m3 were found in QF samples and 62 revertants/m3 in PUF samples. Genotoxic effects of the filter extracts were assessed with the Vitotox assay: some direct genotoxic effects were noted, i.e. without metabolic activation, but almost no effects were observed after metabolic activation. Without activation, most PUF extracts were bacteriotoxic. With metabolic activation this toxicity disappeared, but genotoxic effects were not observed. Statistical analysis showed that the observed biological effects correlated well with the PAH concentrations"
Keywords:		"Air Pollutants/*toxicity Belgium Chromatography, High Pressure Liquid Mutagenicity Tests Mutagens/*toxicity Salmonella typhimurium/genetics;"
Notes:		"MedlineDu Four, V A Van Larebeke, N Janssen, C R eng Research Support, Non-U.S. Gov't Netherlands 2004/03/24 Mutat Res. 2004 Mar 14; 558(1-2):155-67. doi: 10.1016/j.mrgentox.2003.12.002"