| Title: | Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis |
| Author(s): | Dougherty MJ; Hamilton JG; Ward RD; |
| Address: | "Division of Biomedical Sciences, Liverpool School of Tropical Medicine, U.K" |
| DOI: | 10.1111/j.1365-2915.1994.tb00150.x |
| ISSN/ISBN: | 0269-283X (Print) 0269-283X (Linking) |
| Abstract: | "Semiochemical components of eggs of the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) were separated by high performance liquid chromatography. HPLC fractions were examined quantitatively and qualitatively by gas chromatography (GC). A bioassay was used to determine the oviposition attraction of gravid L. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. Gravid flies were placed in individual oviposition tubes to determine if the peak of interest was an oviposition stimulant. The active semiochemical fraction attracted gravid flies for oviposition. Furthermore, egg laying was enhanced: gravid flies exposed to the pheromone oviposited earlier and laid more eggs than control flies. GC analysis indicated that 1200 eggs (2 days old) gave a yield of 12.75 micrograms of active pheromone. This fraction had similar HPLC and GC retention times to caryophyllene oxide, suggesting comparable polarity and molecular weight" |
| Keywords: | "Animals Biological Assay Chromatography, Gas/methods Chromatography, High Pressure Liquid/methods Female *Oviposition/drug effects Ovum/*chemistry Pheromones/chemistry/*isolation & purification/pharmacology Psychodidae/*physiology;" |
| Notes: | "MedlineDougherty, M J Hamilton, J G Ward, R D eng Research Support, Non-U.S. Gov't England 1994/04/01 Med Vet Entomol. 1994 Apr; 8(2):119-24. doi: 10.1111/j.1365-2915.1994.tb00150.x" |
