| Title: | "Effects of permissible maximum-contamination levels of VOC mixture in water on total DNA, antioxidant gene expression, and sequences of ribosomal DNA of Drosophila melanogaster" |
| Author(s): | Doganlar O; Doganlar ZB; Tabakcioglu K; |
| Address: | "Faculty of Medicine, Department of Medical Biology, Trakya University, Edirne, Turkey. doganlar@trakya.edu.tr. Faculty of Medicine, Department of Medical Biology, Trakya University, Edirne, Turkey" |
| Journal Title: | Environ Sci Pollut Res Int |
| DOI: | 10.1007/s11356-015-4741-y |
| ISSN/ISBN: | 1614-7499 (Electronic) 0944-1344 (Linking) |
| Abstract: | "In this study, we aimed to investigate the mutagenic and carcinogenic potential of a volatile organic compound (VOC) mixture with references to the response of D.melanogaster using selected antioxidant gene expressions, RAPD assay and base-pair change of ribosomal 18S, and the internal transcribed spacer, ITS2 rDNA gene sequences. For this purpose, Drosophila melanogaster Oregon R, reared under controlled conditions on artificial diets, were treated with the mixture of thirteen VOCs, which are commonly found in water in concentrations of 10, 20, 50, and 75 ppb for 1 and 5 days. In the random amplified polymorphic DNA (RAPD) assay, band changes were clearly detected, especially at the 50 and 75 ppb exposure levels, for both treatment periods, and the band profiles exhibited clear differences between the treated and untreated flies with changes in band intensity and the loss/appearance of bands. Quantitative real-time PCR (qRT-PCR) analysis of Mn-superoxide dismutase (Mn-SOD), catalase (CAT) and glutathione-synthetase (GS) expressions demonstrated that these markers responded significantly to VOC-induced oxidative stress. Whilst CAT gene expressions increased linearly with increasing concentrations of VOCs and treatment times, the 50- and 75-ppb treatments caused decreases in GS expressions compared to the control at 5 days. Treatment with VOCs at both exposure times, especially in high doses, caused gene mutation of the 18S and the ITS2 ribosomal DNA. According to this research, we thought that the permissible maximum-contamination level of VOCs can cause genotoxic effect especially when mixed" |
| Keywords: | "Animals Catalase/genetics/metabolism DNA Damage DNA, Ribosomal/*genetics Drosophila Proteins/genetics/metabolism Drosophila melanogaster/drug effects/*genetics Gene Expression/drug effects Glutathione Synthase/genetics/metabolism Male Oxidative Stress Ran;" |
| Notes: | "MedlineDoganlar, Oguzhan Doganlar, Zeynep Banu Tabakcioglu, Kiymet eng Research Support, Non-U.S. Gov't Germany 2015/05/29 Environ Sci Pollut Res Int. 2015 Oct; 22(20):15610-20. doi: 10.1007/s11356-015-4741-y. Epub 2015 May 28" |
