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Mol Cell

Title:		Cellular noise suppression by the regulator of G protein signaling Sst2
Author(s):		Dixit G; Kelley JB; Houser JR; Elston TC; Dohlman HG;
Address:		"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: hdohlman@med.unc.edu"
Journal Title:		Mol Cell
Year:		2014
Volume:		20140619
Issue:		1
Page Number:		85 - 96
DOI:		10.1016/j.molcel.2014.05.019
ISSN/ISBN:		1097-4164 (Electronic) 1097-2765 (Print) 1097-2765 (Linking)
Abstract:		"G proteins and their associated receptors process information from a variety of environmental stimuli to induce appropriate cellular responses. Generally speaking, each cell in a population responds within defined limits, despite large variation in the expression of protein signaling components. Therefore, we postulated that noise suppression is encoded within the signaling system. Using the yeast mating pathway as a model, we evaluated the ability of a regulator of G protein signaling (RGS) protein to suppress noise. We found that the RGS protein Sst2 limits variability in transcription and morphogenesis in response to pheromone stimulation. While signal suppression is a result of both the GAP (GTPase accelerating) and receptor binding functions of Sst2, noise suppression requires only the GAP activity. Taken together, our findings reveal a hitherto overlooked role of RGS proteins as noise suppressors and demonstrate an ability to uncouple signal and noise in a prototypical stimulus-response pathway"
Keywords:		"Cell Polarity GTP-Binding Proteins/*metabolism GTPase-Activating Proteins/*physiology Pheromones/metabolism Saccharomyces cerevisiae Proteins/*physiology Signal Transduction Transcription, Genetic cdc42 GTP-Binding Protein, Saccharomyces cerevisiae/metabo;"
Notes:		"MedlineDixit, Gauri Kelley, Joshua B Houser, John R Elston, Timothy C Dohlman, Henrik G eng R01 GM080739/GM/NIGMS NIH HHS/ P30 CA016086/CA/NCI NIH HHS/ GM080739/GM/NIGMS NIH HHS/ GM079271/GM/NIGMS NIH HHS/ R01 GM079271/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2014/06/24 Mol Cell. 2014 Jul 3; 55(1):85-96. doi: 10.1016/j.molcel.2014.05.019. Epub 2014 Jun 19"