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Int J Mol Sci

Title:		Identification and Functional Characterization of Tissue-Specific Terpene Synthases in Stevia rebaudiana
Author(s):		Dhandapani S; Kim MJ; Chin HJ; Leong SH; Jang IC;
Address:		"Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore. Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore"
Journal Title:		Int J Mol Sci
Year:		2020
Volume:		20201113
Issue:		22
Page Number:		-
DOI:		10.3390/ijms21228566
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"In addition to the well-known diterpenoid steviol glycosides, Stevia rebaudiana (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography-Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia TPSs and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of gamma-curcumene/zingiberene/beta-sesquiphellandrene and alpha-longipinene/beta-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the SrTPSs were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue"
Keywords:		"Alkyl and Aryl Transferases/*biosynthesis/genetics Flowers/enzymology/genetics *Gene Expression Regulation, Enzymologic *Gene Expression Regulation, Plant Plant Leaves/enzymology/genetics Plant Proteins/*biosynthesis/genetics Plant Roots/enzymology/geneti;"
Notes:		"MedlineDhandapani, Savitha Kim, Mi Jung Chin, Hui Jun Leong, Sing Hui Jang, In-Cheol eng SBP-P3/National Research Foundation Singapore/ Switzerland 2020/11/19 Int J Mol Sci. 2020 Nov 13; 21(22):8566. doi: 10.3390/ijms21228566"