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Exp Appl Acarol

Title:		Identification and expression of potential olfactory-related genes related to Niemann-Pick C2 protein and ionotropic receptors in Haemaphysalis longicornis
Author(s):		Cui Y; Wang J; Liu Q; Li D; Zhang W; Liu X; Wang J; Song X; Yao F; Wu H; Zhao N;
Address:		"State Key Laboratory of Infectious Disease Prevention and Control, WHO Collaborating Centre for Vector Surveillance and Management, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. College of Agriculture, Shihezi University, Shihezi, Xinjiang, China. State Key Laboratory of Infectious Disease Prevention and Control, WHO Collaborating Centre for Vector Surveillance and Management, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. wuhaixia@icdc.cn. State Key Laboratory of Infectious Disease Prevention and Control, WHO Collaborating Centre for Vector Surveillance and Management, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. zhaoning@icdc.cn"
Journal Title:		Exp Appl Acarol
Year:		2022
Volume:		20220816
Issue:		4
Page Number:		337 - 350
DOI:		10.1007/s10493-022-00729-4
ISSN/ISBN:		1572-9702 (Electronic) 0168-8162 (Linking)
Abstract:		"Studies have shown that the main pathway for tick host localization and perception of mating information may be chemosensory. However, chemical communication in ticks is poorly understood, especially in those other than the Ixodes ticks. Niemann-Pick C2 (NPC2) protein and ionotropic receptors (IRs) are considered to be closely related to the perception of infochemicals in arthropods. Through bioinformatic analysis, eight NPC2 and four IR candidate genes were identified through screening and identification of the transcriptome sequencing database of Haemaphysalis longicornis. Phylogenetic tree analysis indicated that H. longicornis possesses similar homology to the genus Ixodes. A comparison of the expression of NPC2 and IR in tick forelegs (first pair of legs), hind legs (fourth pair of legs), and capitula using RT-PCR revealed that, barring HlonNPC2-8, 11 candidate genes were highly expressed in the foreleg and capitulum, which are the main sensory organs of ticks. They were also expressed in the hind legs, except for six genes that were not expressed in the males. RT-qPCR analysis showed upregulation and higher relative expression of HlonNPC2-1, HlonNPC2-3, HlonNPC2-6, and HlonNPC2-8 when stimulated by ammonium hydroxide, whereas the others were downregulated and demonstrated lower relative expression. These results further support the putative role of NPC2s as a new odorant carrier in ticks and present 12 promising candidate genes for understanding tick olfactory communication, enriching the data on these genes, especially outside the genus Ixodes"
Keywords:		Animals *Arthropods *Ixodes/genetics *Ixodidae/genetics Male Phylogeny Transcriptome Haemaphysalis longicornis Ionotropic receptors Niemann-pick C2 Olfaction;
Notes:		"MedlineCui, Yingying Wang, Jungang Liu, Qiyong Li, Dongmei Zhang, Wen Liu, Xiaobo Wang, Jun Song, Xiuping Yao, Feng Wu, Haixia Zhao, Ning eng 2017ZX10303404-006-002/Special Project for Research and Development in Key areas of Guangdong Province/ 2018ZX10101002-002-011/the National Science and Technology key Projects on Major Infectious Diseases/ Netherlands 2022/08/16 Exp Appl Acarol. 2022 Aug; 87(4):337-350. doi: 10.1007/s10493-022-00729-4. Epub 2022 Aug 16"