| Title: | UCE data reveal multiple origins of rose gallers in North America: Global phylogeny of Diplolepis Geoffroy (Hymenoptera: Cynipidae) |
| Author(s): | Zhang YM; Buffington ML; Looney C; Laszlo Z; Shorthouse JD; Ide T; Lucky A; |
| Address: | "Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, United States; Systematic Entomology Laboratory, USDA-ARS, c/o National Museum of Natural History, Washington DC 20013, United States. Electronic address: yuanmeng.zhang@gmail.com. Systematic Entomology Laboratory, USDA-ARS, c/o National Museum of Natural History, Washington DC 20013, United States. Washington State Department of Agriculture, Olympia, WA 98504, United States. Hungarian Department of Biology and Ecology, Babes-Bolyai University, Cluj-Napoca RO-400006, Romania. Department of Biology, Laurentian University, Sudbury, Ontario P3E 2C6, Canada. Department of Zoology, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba, Ibaraki 305-0005, Japan. Entomology and Nematology Department, University of Florida, Gainesville, FL 32611, United States" |
| DOI: | 10.1016/j.ympev.2020.106949 |
| ISSN/ISBN: | 1095-9513 (Electronic) 1055-7903 (Linking) |
| Abstract: | "Gall wasps in the genus Diplolepis Geoffroy are specialized herbivores that induce galls exclusively on roses. Despite their wide distribution across the Holarctic, little is known about their evolutionary history. Here we present the first phylogenomic tree of global Diplolepis reconstructed using Ultraconserved Elements (UCEs), resulting in a robust phylogeny based on 757 genes. Results support the existence of two principal clades: a Nearctic stem-galler clade, and a Holarctic leaf-galler clade that further splits into two Palearctic groups and one Nearctic group. This topology is congruent with a previous study based on the mitochondrial gene COI, an unexpected result given the common occurrence of mitonuclear discordance in closely related oak gall wasp lineages. Most Diplolepis species were recovered as reciprocally monophyletic, with some notable exceptions such as the D. polita and the D. ignota complex, for which species boundaries remain unresolved. Historical biogeographic reconstruction was unable to pinpoint the origin of Diplolepis, but confirms two independent incursions into the Nearctic. Ancestral state reconstruction analysis highlights the conservatism of gall location on the host plants, as shifts to different host organs are relatively rare. We suggest that Diplolepis were originally leaf gallers, with a Nearctic stem-galler clade undergoing a major plant organ switch onto rose stems. Host organ switch or reversal is uncommon, which suggests a level of conservatism. Our study showcases the resolving power of UCEs at the species level while also suggesting improvements to advance future Cynipoidea phylogenomics. Our results also highlight the additional sampling needed to clarify taxonomic relationships in the Nearctic and eastern Palearctic regions" |
| Keywords: | "Animals Conserved Sequence/genetics Genes, Mitochondrial/genetics North America *Phylogeny Rosa/parasitology Wasps/*classification/*genetics Ancestral state reconstruction Biogeography Gall wasp Phylogenomic Target capture Ultraconserved elements;" |
| Notes: | "MedlineZhang, Y Miles Buffington, Matthew L Looney, Chris Laszlo, Zoltan Shorthouse, Joseph D Ide, Tatsuya Lucky, Andrea eng Research Support, Non-U.S. Gov't 2020/09/01 Mol Phylogenet Evol. 2020 Dec; 153:106949. doi: 10.1016/j.ympev.2020.106949. Epub 2020 Aug 29" |
