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Mol Cell Biol

Title:		Schizosaccharomyces pombe map1+ encodes a MADS-box-family protein required for cell-type-specific gene expression
Author(s):		Yabana N; Yamamoto M;
Address:		"Department of Biophysics and Biochemistry, School of Science, University of Tokyo, Hongo, Japan"
Journal Title:		Mol Cell Biol
Year:		1996
Volume:		16
Issue:		7
Page Number:		3420 - 3428
DOI:		10.1128/MCB.16.7.3420
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"We cloned the Schizosaccharomyces pombe map1 gene by virtue of its ability to stimulate transcription of the sxa2 gene, which encodes a carboxypeptidase expressed specifically in h- cells in response to mating-pheromone signaling. The cloned gene had a coding capacity of 398 amino acids split by two introns, and the deduced product was a protein of the MADS box family. This gene was most similar to Saccharomyces cerevisiae MCM1, which regulates cell-type-specific gene expression in budding yeast cells. Disruption of the S. pombe gene did not affect vegetative cell growth but conferred sterility. It blocked the mating ability of h+ cells completely and that of h- cells partially. Genetic and sequencing analysis indicated that the cloned gene is map1], which was originally defined by a mutation that caused h+-speciftic sterility. Northern (RNA) blot analysis showed that the function of map1 is absolutely essential for the expression of h+-specific genes and is required for the full activation of h--specific gene expression. Overexpression of map1 resulted in enhanced transcription of cell-type-specilic genes, but the range of genes affected by Map1 was restricted by the mating type of the cell. Results of yeast two-hybrid analysis suggested that Map1 may physically interact with Mat1-Pc, the product of the h(+)-specific mating-type gene mat1-Pc. On the basis of these observations, we speculate that Map1 may be a transcriptional regulator of cell-type-specific genes similar to S. cerevisiae MCM1, whose activity is modulated by the oil and alpha2 mating-type gene products"
Keywords:		"Amino Acid Sequence Base Sequence Cloning, Molecular DNA Primers *Gene Expression Gene Expression Regulation, Fungal Genes, Fungal Genes, Mating Type, Fungal Genotype Histidine/metabolism Homeodomain Proteins/*biosynthesis/genetics Humans Molecular Sequen;"
Notes:		"MedlineYabana, N Yamamoto, M eng Comparative Study Research Support, Non-U.S. Gov't 1996/07/01 Mol Cell Biol. 1996 Jul; 16(7):3420-8. doi: 10.1128/MCB.16.7.3420"