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Animal

Title:		Thiamine modulates intestinal morphological structure and microbiota under subacute ruminal acidosis induced by a high-concentrate diet in Saanen goats
Author(s):		Wen K; Zhao MM; Liu L; Khogali MK; Geng TY; Wang HR; Gong DQ;
Address:		"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu Province, PR China. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu Province, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, PR China. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu Province, PR China; Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu Province, PR China. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu Province, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, PR China. Electronic address: yzgong@163.com"
Journal Title:		Animal
Year:		2021
Volume:		20210925
Issue:		10
Page Number:		100370 -
DOI:		10.1016/j.animal.2021.100370
ISSN/ISBN:		1751-732X (Electronic) 1751-7311 (Linking)
Abstract:		"Ruminant animals are generally fed with starch-rich grain as the main energy source, and the incidence of metabolic diseases such as subacute ruminal acidosis (SARA) is high due to the intensive farming. Thiamin has been reported to alleviate SARA caused by high-concentrate diets, but the exact mechanism is not well understood. The goal of this study was to examine the role of thiamine in intestinal inflammation and microbiota caused by high-concentrate diets. The SARA model was induced by low neutral detergent fibre/starch ration to study the effects of thiamine on intestinal tissue structure and microbiota. 18 mid-lactation (148 +/- 3 d in milk; milk yield = 0.71 +/- 0.0300 kg/d) Saanen goats (BW = 36.5 +/- 1.99 kg; body condition score = 2.73 +/- 0.16, where 1 = emaciated and 6 = obese) in parities 1 or 2 were selected. The goats were randomly divided into three groups with six replicates: (1) control diet (C; concentrate:forage 30:70), (2) high-concentrate diet (H; concentrate:forage 70:30), and (3) high-concentrate diet with 200 mg of thiamine/kg of DM intake (H + T;concentrate:forage 70:30). The experimental period was lasted for 56 d. The small and large intestine, expression of inflammatory factor genes, tight junction protein genes, total antioxidant capacity, and intestinal microbiota were measured. The results showed that SARA was observed in treatment H, whereas rumen fluid pH was improved in treatment H + T. Treatment H + T also significantly repaired the intestinal tissue structure damaged by SARA, improved the total antioxidant capacity of the small intestinal mucosa, reduced mRNA expression of inflammatory factors in the small intestine tissue, and increased the mRNA expression of tight junction genes in small intestine tissue. The high-concentrate diet reduced the diversity of intestinal microbiota. When thiamine is added to the high-concentrate diet, the relative abundance of intestinal Firmicutes and beneficial bacteria represented by Lactobacilli were upregulated, and the relative abundance of Proteus, a marker of intestinal dysbacteriosis, returned to normal. In conclusion, thiamine supplementation could alleviate the damage to the intestinal tissue structure and microbial environment caused by SARA condition in dairy goats fed a high-concentrate diet"
Keywords:		*Acidosis/veterinary Animals Cattle *Cattle Diseases Diet/veterinary Female *Goat Diseases Goats Hydrogen-Ion Concentration Lactation *Microbiota Milk Rumen Thiamine 16S rRNA Dairy goat Intestinal tissues Metabolic diseases Vitamin B(1);Animals;
Notes:		"MedlineWen, K Zhao, M M Liu, L Khogali, Mawahib K Geng, T Y Wang, H R Gong, D Q eng Randomized Controlled Trial, Veterinary England 2021/09/29 Animal. 2021 Oct; 15(10):100370. doi: 10.1016/j.animal.2021.100370. Epub 2021 Sep 25"