| Title: | p38 mitogen-activated protein kinase/Hog1p regulates translation of the AU-rich-element-bearing MFA2 transcript |
| Author(s): | Vasudevan S; Garneau N; Tu Khounh D; Peltz SW; |
| Address: | "Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School and Rutgers University, 675 Hoes Lane, Piscataway, NJ 08854, USA" |
| DOI: | 10.1128/MCB.25.22.9753-9763.2005 |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "AU-rich-element (ARE)-mediated mRNA regulation occurs in Saccharomyces cerevisiae in response to external and internal stimuli through the p38 mitogen-activated protein kinase (MAPK)/Hog1p pathway. We demonstrate that the ARE-bearing MFA2 3' untranslated region (UTR) controls translation efficiency in a p38 MAPK/Hog1p-dependent manner in response to carbon source growth conditions. The carbon source-regulated effect on MFA2 3'-UTR-controlled translation involves the role of conserved ARE binding proteins, the ELAV/TIA-1-like Pub1p, which can interact with the cap/eIF4G complex, and the translation/mRNA stability factor poly(A) binding protein (Pab1p). Pub1p binds the MFA2 3'-UTR in a p38 MAPK/Hog1p-regulated manner in response to carbon source growth conditions. Significantly, the p38 MAPK/Hog1p is also required to modulate Pab1p in response to carbon source. We find that Pab1p can bind the MFA2 3'-UTR in a regulated manner to control MFA2 3'-UTR reporter translation. Binding of full-length Pab1p to the MFA2 3'-UTR correlates with translation repression. Importantly, Pab1p binds the MFA2 3'-UTR only in a PUB1 strain, and correlating with this requirement, Pub1p controls translation repression of MFA2 in a carbon source/Hog1p-regulated manner. These results suggest that the p38 MAPK/Hog1p pathway regulates 3'-UTR-mediated translation by modulating recruitment of Pab1p and Pub1p, which can interact with the translation machinery" |
| Keywords: | "3' Untranslated Regions Carbon/pharmacology Cross-Linking Reagents/pharmacology DNA/chemistry Fungal Proteins/*metabolism *Gene Expression Regulation Genes, Fungal Genes, Reporter Genotype Glucose/pharmacology Glycerol/pharmacology Lipoproteins/*physiolog;" |
| Notes: | "MedlineVasudevan, Shobha Garneau, Nicole Tu Khounh, Danny Peltz, Stuart W eng R01 GM058276/GM/NIGMS NIH HHS/ GM58276/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S. 2005/11/02 Mol Cell Biol. 2005 Nov; 25(22):9753-63. doi: 10.1128/MCB.25.22.9753-9763.2005" |
