| Title: | The rice hydroperoxide lyase OsHPL3 functions in defense responses by modulating the oxylipin pathway |
| Author(s): | Tong X; Qi J; Zhu X; Mao B; Zeng L; Wang B; Li Q; Zhou G; Xu X; Lou Y; He Z; |
| Address: | "College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China" |
| DOI: | 10.1111/j.1365-313X.2012.05027.x |
| ISSN/ISBN: | 1365-313X (Electronic) 0960-7412 (Linking) |
| Abstract: | "As important signal molecules, jasmonates (JAs) and green leaf volatiles (GLVs) play diverse roles in plant defense responses against insect pests and pathogens. However, how plants employ their specific defense responses by modulating the levels of JA and GLVs remains unclear. Here, we describe identification of a role for the rice HPL3 gene, which encodes a hydroperoxide lyase (HPL), OsHPL3/CYP74B2, in mediating plant-specific defense responses. The loss-of-function mutant hpl3-1 produced disease-resembling lesions spreading through the whole leaves. A biochemical assay revealed that OsHPL3 possesses intrinsic HPL activity, hydrolyzing hydroperoxylinolenic acid to produce GLVs. The hpl3-1 plants exhibited enhanced induction of JA, trypsin proteinase inhibitors and other volatiles, but decreased levels of GLVs including (Z)-3-hexen-1-ol. OsHPL3 positively modulates resistance to the rice brown planthopper [BPH, Nilaparvata lugens (Stal)] but negatively modulates resistance to the rice striped stem borer [SSB, Chilo suppressalis (Walker)]. Moreover, hpl3-1 plants were more attractive to a BPH egg parasitoid, Anagrus nilaparvatae, than the wild-type, most likely as a result of increased release of BPH-induced volatiles. Interestingly, hpl3-1 plants also showed increased resistance to bacterial blight (Xanthomonas oryzae pv. oryzae). Collectively, these results indicate that OsHPL3, by affecting the levels of JA, GLVs and other volatiles, modulates rice-specific defense responses against different invaders" |
| Keywords: | Aldehyde-Lyases/*metabolism Animals Cyclopentanes/metabolism Cytochrome P-450 Enzyme System/*metabolism Female *Herbivory Insecta/*physiology Mutation Oryza/enzymology/microbiology/*physiology Oxylipins/*metabolism Plant Proteins/metabolism Salicylic Acid; |
| Notes: | "MedlineTong, Xiaohong Qi, Jinfeng Zhu, Xudong Mao, Bizeng Zeng, Longjun Wang, Baohui Li, Qun Zhou, Guoxin Xu, Xiaojing Lou, Yonggen He, Zuhua eng Research Support, Non-U.S. Gov't England 2012/04/24 Plant J. 2012 Sep; 71(5):763-75. doi: 10.1111/j.1365-313X.2012.05027.x. Epub 2012 Jun 18" |
