| Title: | Repression of jasmonate signaling by a non-TIFY JAZ protein in Arabidopsis |
| Author(s): | Thireault C; Shyu C; Yoshida Y; St Aubin B; Campos ML; Howe GA; |
| Address: | "Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA" |
| ISSN/ISBN: | 1365-313X (Electronic) 0960-7412 (Linking) |
| Abstract: | "JAsmonate ZIM-domain (JAZ) proteins repress the activity of transcription factors that execute responses to the plant hormone jasmonoyl-L-isoleucine (JA-Ile). The ZIM protein domain recruits the co-repressors NINJA and TOPLESS to JAZ-bound transcription factors, and contains a highly conserved TIF[F/Y]XG motif that defines the larger family of TIFY proteins to which JAZs belong. Here, we report that diverse plant species contain genes encoding putative non-TIFY JAZ proteins, including a previously unrecognized JAZ repressor in Arabidopsis (JAZ13, encoded by At3g22275). JAZ13 is most closely related to JAZ8 and includes divergent EAR, TIFY/ZIM, and Jas motifs. Unlike JAZ8, however, JAZ13 contains a Ser-rich C-terminal tail that is a site for phosphorylation. Overexpression of JAZ13 resulted in reduced sensitivity to JA, attenuation of wound-induced expression of JA-response genes, and decreased resistance to insect herbivory. JAZ13 interacts with the bHLH transcription factor MYC2 and the co-repressor TOPLESS but, consistent with the absence of a TIFY motif, neither NINJA nor other JAZs. Analysis of single and higher-order T-DNA insertion jaz null mutants provided further evidence that JAZ13 is a repressor JA signaling. Our results demonstrate that proteins outside the TIFY family are functional JAZ repressors and further suggest that this expansion of the JAZ family allows fine-tuning of JA-mediated transcriptional responses" |
| Keywords: | "Arabidopsis/genetics/*metabolism Cyclopentanes/metabolism Gene Expression Regulation, Plant Isoleucine/analogs & derivatives/metabolism Oxylipins/metabolism Plant Growth Regulators/metabolism Arabidopsis thaliana JAsmonate-ZIM TIFY protein hormone signali;" |
| Notes: | "MedlineThireault, Caitlin Shyu, Christine Yoshida, Yuki St Aubin, Brian Campos, Marcelo L Howe, Gregg A eng R01 GM57795/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2015/04/08 Plant J. 2015 May; 82(4):669-79. doi: 10.1111/tpj.12841" |
