| Title: | Online breath gas analysis in unrestrained mice by hs-PTR-MS |
| Author(s): | Szymczak W; Rozman J; Hollriegl V; Kistler M; Keller S; Peters D; Kneipp M; Schulz H; Hoeschen C; Klingenspor M; de Angelis MH; |
| Address: | "Research Unit Medical Radiation Physics and Diagnostics, Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 85764, Neuherberg, Germany, szymczak@helmholtz-muenchen.de" |
| DOI: | 10.1007/s00335-013-9493-8 |
| ISSN/ISBN: | 1432-1777 (Electronic) 0938-8990 (Linking) |
| Abstract: | "The phenotyping of genetic mouse models for human disorders may greatly benefit from breath gas analysis as a noninvasive tool to identify metabolic alterations in mice. Phenotyping screens such as the German Mouse Clinic demand investigations in unrestrained mice. Therefore, we adapted a breath screen in which exhaled volatile organic compounds (VOCs) were online monitored by proton transfer reaction mass spectrometry (hs-PTR-MS). The source strength of VOCs was derived from the dynamics in the accumulation profile of exhaled VOCs of a single mouse in a respirometry chamber. A careful survey of the accumulation revealed alterations in the source strength due to confounders, e.g., urine and feces. Moreover changes in the source strength of humidity were triggered by changes in locomotor behavior as mice showed a typical behavioral pattern from activity to settling down in the course of subsequent accumulation profiles. We demonstrated that metabolic changes caused by a dietary intervention, e.g., after feeding a high-fat diet (HFD) a sample of 14 male mice, still resulted in a statistically significant shift in the source strength of exhaled VOCs. Applying a normalization which was derived from the distribution of the source strength of humidity and accounted for varying locomotor behaviors improved the shift. Hence, breath gas analysis may provide a noninvasive, fast access to monitor the metabolic adaptation of a mouse to alterations in energy balance due to overfeeding or fasting and dietary macronutrient composition as well as a high potential for systemic phenotyping of mouse mutants, intervention studies, and drug testing in mice" |
| Keywords: | "Animals Breath Tests/instrumentation/*methods Cluster Analysis Diet, High-Fat *Disease Models, Animal Energy Metabolism/*physiology Mass Spectrometry/*methods Metabolic Networks and Pathways/*physiology Mice Mice, Inbred C57BL Volatile Organic Compounds/a;" |
| Notes: | "MedlineSzymczak, Wilfried Rozman, Jan Hollriegl, Vera Kistler, Martin Keller, Stefan Peters, Dominika Kneipp, Moritz Schulz, Holger Hoeschen, Christoph Klingenspor, Martin de Angelis, Martin Hrabe eng Research Support, Non-U.S. Gov't 2013/11/28 Mamm Genome. 2014 Apr; 25(3-4):129-40. doi: 10.1007/s00335-013-9493-8. Epub 2013 Nov 26" |
