| Title: | Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus |
| Author(s): | Shi XB; Wang XZ; Zhang DY; Zhang ZH; Zhang Z; Cheng J; Zheng LM; Zhou XG; Tan XQ; Liu Y; |
| Address: | "Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. xiaobin.s@163.com. Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. shirley86111@163.com. Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. dyzhang78@163.com. Hunan Academy of Agricultural Sciences, Institute of Vegetable, Changsha 410000, China. zhangzhanhong@huncaas.cn. Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. zhangzhuo@hunaas.cn. Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. lmzheng66@126.com. Department of Entomology, University of Kentucky, Lexington, KY 40546, USA. xuguozhou@uky.edu. Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. tanxinqiu2008@163.com. Hunan Academy of Agricultural Sciences, Institute of Plant Protection, Changsha 410000, China. haoasliu@163.com" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Tomato chlorosis virus (ToCV) is widespread, seriously impacting tomato production throughout the world. ToCV is semi-persistently transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Currently, insect olfaction is being studied to develop novel pest control technologies to effectively control B. tabaci and whitefly-borne virus diseases. Despite current research efforts, no report has been published on the role of odorant-binding proteins (OBPs) in insect preference under the influence of plant virus. Our previous research showed that viruliferous B. tabaci preferred healthy plants at 48 h after virus acquisition. In this study, we determined the effect of OBPs on the host preference interactions of ToCV and whiteflies. Our results show that with the increase in acquisition time, the OBP gene expressions changed differently, and the OBP3 gene expression showed a trend of first rising and then falling, and reached the maximum at 48 h. These results indicate that OBP3 may participate in the host preference of viruliferous whiteflies to healthy plants. When the expression of the OBP3 gene was knocked down by an RNA interference (RNAi) technique, viruliferous Mediterranean (MED) showed no preference and the ToCV transmission rate was reduced by 83.3%. We conclude that OBP3 is involved in the detection of plant volatiles by viruliferous MED. Our results provide a theoretical basis and technical support for clarifying the transmission mechanism of ToCV by B. tabaci and could provide new avenues for controlling this plant virus and its vectors" |
| Keywords: | "Animals Crinivirus/*physiology Disease Transmission, Infectious *Gene Silencing Genes, Reporter Hemiptera/virology Host-Pathogen Interactions/genetics Insect Vectors/*genetics/*virology Solanum lycopersicum/virology Plant Diseases/virology *RNA Interferen;" |
| Notes: | "MedlineShi, Xiao-Bin Wang, Xue-Zhong Zhang, De-Yong Zhang, Zhan-Hong Zhang, Zhuo Cheng, Ju'E Zheng, Li-Min Zhou, Xu-Guo Tan, Xin-Qiu Liu, Yong eng 2017YFD0200900/the National Key R&D Program of China/ CARS-23-D-02/the Agriculture Research System of China/ 31872932, 31571981, 31571982, 31672003/the National Natural Science Foundation of China/ 2019JJ30014/Hunan Natural Science Foundation/ 2016RS2019/Hunan talent project/ Switzerland 2019/10/12 Int J Mol Sci. 2019 Oct 9; 20(20):4969. doi: 10.3390/ijms20204969" |
