| Title: | Quantitative analysis of the yeast pheromone pathway |
| Author(s): | Shellhammer JP; Pomeroy AE; Li Y; Dujmusic L; Elston TC; Hao N; Dohlman HG; |
| Address: | "Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. Division of Biological Sciences, University of California San Diego, San Diego, CA, 92093, USA" |
| ISSN/ISBN: | 1097-0061 (Electronic) 0749-503X (Print) 0749-503X (Linking) |
| Abstract: | "The pheromone response pathway of the yeast Saccharomyces cerevisiae is a well-established model for the study of G proteins and mitogen-activated protein kinase (MAPK) cascades. Our longstanding ability to combine sophisticated genetic approaches with established functional assays has provided a thorough understanding of signalling mechanisms and regulation. In this report, we compare new and established methods used to quantify pheromone-dependent MAPK phosphorylation, transcriptional induction, mating morphogenesis, and gradient tracking. These include both single-cell and population-based assays of activity. We describe several technical advances, provide example data for benchmark mutants, highlight important differences between newer and established methodologies, and compare the advantages and disadvantages of each as applied to the yeast model. Quantitative measurements of pathway activity have been used to develop mathematical models and reveal new regulatory mechanisms in yeast. It is our expectation that experimental and computational approaches developed in yeast may eventually be adapted to human systems biology and pharmacology" |
| Keywords: | Mitogen-Activated Protein Kinases/genetics/metabolism Morphogenesis Mutation Pheromones/genetics/*metabolism Phosphorylation Saccharomyces cerevisiae/genetics/*metabolism Saccharomyces cerevisiae Proteins/genetics/metabolism *Signal Transduction Single-Ce; |
| Notes: | "MedlineShellhammer, James P Pomeroy, Amy E Li, Yang Dujmusic, Lorena Elston, Timothy C Hao, Nan Dohlman, Henrik G eng R01 GM114136/GM/NIGMS NIH HHS/ R35 GM127145/GM/NIGMS NIH HHS/ R01GM114136/GF/NIH HHS/ P30 CA016086/CA/NCI NIH HHS/ R01GM111458/GF/NIH HHS/ R01 GM111458/GM/NIGMS NIH HHS/ R35GM118105/GF/NIH HHS/ R35 GM118105/GM/NIGMS NIH HHS/ Comparative Study Research Support, N.I.H., Extramural England 2019/04/26 Yeast. 2019 Aug; 36(8):495-518. doi: 10.1002/yea.3395. Epub 2019 Jun 27" |
