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Microbiol Spectr

Title:		Volatile Organic Compounds Emitted by the Biocontrol Agent Pythium oligandrum Contribute to Ginger Plant Growth and Disease Resistance
Author(s):		Sheikh TMM; Zhou D; Ali H; Hussain S; Wang N; Chen S; Zhao Y; Wen X; Wang X; Zhang J; Wang L; Deng S; Feng H; Raza W; Fu P; Peng H; Wei L; Daly P;
Address:		"Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China. School of Biosciences, University of Birmingham, Birmingham, United Kingdom. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing, China. Fungal Genomics Laboratory (FungiG), Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, China. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, China. Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China. Jiangsu Coastal Ecological Science and Technology Development Co., Ltd., Nanjing, China"
Journal Title:		Microbiol Spectr
Year:		2023
Volume:		20230803
Issue:		4
Page Number:		e0151023 -
DOI:		10.1128/spectrum.01510-23
ISSN/ISBN:		2165-0497 (Electronic) 2165-0497 (Linking)
Abstract:		"The oomycete Pythium oligandrum is a potential biocontrol agent to control a wide range of fungal and oomycete-caused diseases, such as Pythium myriotylum-caused rhizome rot in ginger, leading to reduced yields and compromised quality. Previously, P. oligandrum has been studied for its plant growth-promoting potential by auxin production and induction of disease resistance by elicitors such as oligandrin. Volatile organic compounds (VOCs) play beneficial roles in sustainable agriculture by enhancing plant growth and resistance. We investigated the contribution of P. oligandrum-produced VOCs on plant growth and disease suppression by initially using Nicotiana benthamiana plants for screening. P. oligandrum VOCs significantly enhanced tobacco seedling and plant biomass contents. Screening of the individual VOCs showed that 3-octanone and hexadecane promoted the growth of tobacco seedlings. The total VOCs from P. oligandrum also enhanced the shoot and root growth of ginger plants. Transcriptomic analysis showed a higher expression of genes related to plant growth hormones and stress responses in the leaves of ginger plants exposed to P. oligandrum VOCs. The concentrations of plant growth hormones such as auxin, zeatin, and gibberellic acid were higher in the leaves of ginger plants exposed to P. oligandrum VOCs. In a ginger disease biocontrol assay, the VOC-exposed ginger plants infected with P. myriotylum had lower levels of disease severity. We conclude that this study contributes to understanding the growth-promoting mechanisms of P. oligandrum on ginger and tobacco, priming of ginger plants against various stresses, and the mechanisms of action of P. oligandrum as a biocontrol agent. IMPORTANCE Plant growth promotion plays a vital role in enhancing production of agricultural crops, and Pythium oligandrum is known for its plant growth-promoting potential through production of auxins and induction of resistance by elicitors. This study highlights the significance of P. oligandrum-produced VOCs in plant growth promotion and disease resistance. Transcriptomic analyses of leaves of ginger plants exposed to P. oligandrum VOCs revealed the upregulation of genes involved in plant growth hormone signaling and stress responses. Moreover, the concentration of growth hormones significantly increased in P. oligandrum VOC-exposed ginger plants. Additionally, the disease severity was reduced in P. myriotylum-infected ginger plants exposed to P. oligandrum VOCs. In ginger, P. myriotylum-caused rhizome rot disease results in severe losses, and biocontrol has a role as part of an integrated pest management strategy for rhizome rot disease. Overall, growth enhancement and disease reduction in plants exposed to P. oligandrum-produced VOCs contribute to its role as a biocontrol agent"
Keywords:		*Pythium/genetics *Volatile Organic Compounds/pharmacology *Ginger/microbiology Disease Resistance Tobacco Plant Diseases/prevention & control/microbiology Pythium myriotylum Pythium oligandrum Zingiber officinale disease severity ginger plant growth prom;
Notes:		"MedlineSheikh, Taha Majid Mahmood Zhou, Dongmei Ali, Haider Hussain, Sarfraz Wang, Nan Chen, Siqiao Zhao, Yishen Wen, Xian Wang, Xiaoyu Zhang, Jinfeng Wang, Lunji Deng, Sheng Feng, Hui Raza, Waseem Fu, Pengxiao Peng, Hao Wei, Lihui Daly, Paul eng Research Support, Non-U.S. Gov't 2023/08/03 Microbiol Spectr. 2023 Aug 17; 11(4):e0151023. doi: 10.1128/spectrum.01510-23. Epub 2023 Aug 3"