| Title: | Rapid screening and quantification of major organic acids in citrus fruits and their bioactivity studies |
| Author(s): | Sharma R; Verma S; Rana S; Rana A; |
| Address: | "Food and Nutraceuticals Division, CSIR-HRDC Campus, Ghaziabad, Uttar Pradesh 201 002 India. 2Academy of Scientific and Innovative Research (AcSIR), Chennai, India. GRID: grid.469887.c 3Department of Food Science, Nutrition and Technology, College of Home Science, CSK HPKV, Palampur, Himachal Pradesh India. ISNI: 0000 0000 8733 2729. GRID: grid.411939.7" |
| DOI: | 10.1007/s13197-018-3045-x |
| ISSN/ISBN: | 0022-1155 (Print) 0975-8402 (Electronic) 0022-1155 (Linking) |
| Abstract: | "Organic acids (OAs) are small non-volatile molecules with widespread usage in processed foods, feeds and instant beverages. The prime aim of this study was to explore major OAs in local citrus fruits (Citrus limetta, Citrus aurantifolia, Citrus nobilis, Citrus karna, Citrus medica, Citrus ichangensis and Citrus aurantium) and assessment of their bioactivities. A RP-HPLC-DAD method was developed using buffer free solvent system for rapid detection and quantification of major OAs from citrus fruits and derived products. Method validation studies showed good linear calibration curve (0.985-0.998) for all OAs. The values of %RSD ranged between 0.0001-1.129 and 0.142-1.941 for interday and intraday variability respectively. The limit of detection and limit of quantification values for different OAs were ranged between 1.5-12 and 5-40 microg mL(-1). The juice of above mentioned citrus fruit cultivars were assessed for OAs, total phenolics, free radical scavenging antioxidants and their antimicrobial potential against selected bacterial and fungal strains. The results showed variable contents of phenolics [0.28 +/- 0.001-1.17 +/- 0.014 mg (GAE) mL(-1)] and antioxidant compounds (1.26 +/- 0.009-2.84 +/- 0.006 mg of trolox equivalents mL(-1)) in all juice samples besides significant antifungal activity against C. albicans and A. niger strains. However, in case of antibacterial activity, only C. aurantifolia showed inhibitory effects against selected strains. It was found that citrus fruits have immense potential for their utilization as economic source of natural OAs and development of value added products, beverages and bio-preservatives" |
| Keywords: | Antimicrobial activity Antioxidants Citrus fruits Organic acids Phenolics Rp-hplc; |
| Notes: | "PubMed-not-MEDLINESharma, Ranjana Verma, Sarika Rana, Shalika Rana, Ajay eng India 2018/04/03 J Food Sci Technol. 2018 Apr; 55(4):1339-1349. doi: 10.1007/s13197-018-3045-x. Epub 2018 Jan 29" |
