| Title: | Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies |
| Address: | "Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, Hans-Knoll-Str.8, 07745, Jena, Germany. Research & Development, KWS SAAT SE, Grimsehlstrasse 31, 37574, Einbeck, Germany. stefan.meldau@kws.com" |
| DOI: | 10.1007/978-1-4939-6831-2_10 |
| ISSN/ISBN: | 1940-6029 (Electronic) 1064-3745 (Linking) |
| Abstract: | "Although many established tools for cytokinin (CK) pathway manipulations are well suitable for the analysis of molecular interactions, their use on a whole plant scale is often limited by the induction of severe developmental defects. To circumvent this problem, different methods were developed that allow for a more precise manipulation of the CK pathway. Here we present one of these systems, the pOp6/LhGR system for chemically inducible gene expression. This system allows regulation on a spatial, temporal, and quantitative scale and therefore provides a superior tool for analyzing the role of CKs in the interactions of plants with their environment. The pOp6/LhGR system was tested for RNAi-mediated gene silencing and heterologous gene expression and was successfully used for CK pathway manipulations in different model organisms (Arabidopsis thaliana, Nicotiana tabaccum, Nicotiana attenuata, Citrus sinensis x C. trifoliate). Here we describe specific aspects of the screening procedure and present an experimental setup that can not only be used in the laboratory but is also applicable under field conditions" |
| Keywords: | "Cytokinins/*metabolism Gene Expression Regulation, Plant *Genetic Engineering Genetic Testing Herbivory Phenotype Plants/*genetics/*metabolism Plants, Genetically Modified *Signal Transduction Cytokinins Dexamethasone Ecology Fieldwork Isopentenyl transfe;" |
| Notes: | "MedlineSchafer, Martin Meldau, Stefan eng 2017/03/08 Methods Mol Biol. 2017; 1569:127-139. doi: 10.1007/978-1-4939-6831-2_10" |
