| Title: | Trichoderma harzianum Volatile Organic Compounds Regulated by the THCTF1 Transcription Factor Are Involved in Antifungal Activity and Beneficial Plant Responses |
| Author(s): | Rubio MB; Monti MM; Gualtieri L; Ruocco M; Hermosa R; Monte E; |
| Address: | "Department of Microbiology and Genetics, Institute for Agribiotechnology Research (CIALE), University of Salamanca, Campus de Villamayor, C/Duero, 12, 37185 Salamanca, Spain. Institute for Sustainable Plant Protection (CNR-IPSP), Piazzale Enrico Fermi 1, 80055 Naples, Italy" |
| ISSN/ISBN: | 2309-608X (Electronic) 2309-608X (Linking) |
| Abstract: | "The transcription factor THCTF1 from Trichoderma harzianum, previously linked to the production of 6-pentyl-2H-pyran-2-one (6-PP) derivatives and antifungal activity against Fusarium oxysporum, has been related in this study to conidiation, production of an array of volatile organic compounds (VOCs) and expression of methyltransferase genes. VOCs emitted by three T. harzianum strains (wild type T34, transformant DeltaD1-38 that is disrupted in the Thctf1 gene encoding the transcription factor THCTF1, and ectopic integration transformant DeltaJ3-16) were characterized by Proton Transfer Reaction-Quadrupole interface-Time-Of-Flight-Mass Spectrometry (PTR-Qi-TOF-MS). Thctf1 disruption affected the production of numerous VOCs such as the antifungal volatiles 2-pentyl furan and benzaldehyde which were under-emitted, and acetoine, a plant systemic defense inductor, which was over-emitted. Biological assays show that VOCs regulated by THCTF1 are involved in the T. harzianum antifungal activity against Botrytis cinerea and in the beneficial effects leading to Arabidopsis plant development. The VOC blend from the disruptant DeltaD1-38: (i) inhibited Arabidopsis seed germination for at least 26 days and (ii) when applied to Arabidopsis seedlings resulted in increased jasmonic acid- and salicylic acid-dependent defenses" |
| Keywords: | Botrytis cinerea Ja PTR-Qi-TOF-MS Sa VOCs gene disruption methyltransferase plant growth promotion plant immunity volatilome; |
| Notes: | "PubMed-not-MEDLINERubio, Maria Belen Monti, Maurilia Maria Gualtieri, Liberata Ruocco, Michelina Hermosa, Rosa Monte, Enrique eng PID-2021-126575OB-I00 and TED2021-130934B-I00/Spanish MCIN/AEI/ 10.13039/501100011033/ SA094P20,CLU-2018-04 and IR2020-1-USAL05/Castile and Leon/ Switzerland 2023/06/27 J Fungi (Basel). 2023 Jun 11; 9(6):654. doi: 10.3390/jof9060654" |
