| Title: | Specific Metabolites in a Phaeodactylum tricornutum Strain Isolated from Western Norwegian Fjord Water |
| Author(s): | Prestegard SK; Erga SR; Steinrucken P; Mjos SA; Knutsen G; Rohloff J; |
| Address: | "Bergen Marine Biobank, Department of Biology, University of Bergen, Thormohlensgate 53A/B, N-5020 Bergen, Norway. siv.prestegard@uni.no. Applied Biotechnology, Uni Research Environment, Thormohlensgate 49B, N-5006 Bergen, Norway. siv.prestegard@uni.no. Department of Biology, University of Bergen, Thormohlensgate 53AB, N-5020 Bergen, Norway. svein.erga@uib.no. Department of Biology, University of Bergen, Thormohlensgate 53AB, N-5020 Bergen, Norway. pia.steinrucken@uib.no. Department of Chemistry, University of Bergen, Allegaten 42, N-5020 Bergen, Norway. svein.mjos@uib.no. Department of Biology, University of Bergen, Thormohlensgate 53AB, N-5020 Bergen, Norway. gjert.knutsen@uib.no. Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway. jens.rohloff@ntnu.no" |
| ISSN/ISBN: | 1660-3397 (Electronic) 1660-3397 (Linking) |
| Abstract: | "We have searched for special characteristics in growth, protein expression, fatty acids and volatile organic compounds (VOCs) in a local Phaeodactylum tricornutum Bohlin strain (Bergen Marine Biobank), by comparing it with a common accession strain (CCAP). Differences in growth and expressed proteins were detected between the BMB strain and the CCAP strain, and the BMB strain reached the highest cell densities under the given growth conditions. Fatty acid (FA) analyses showed highest relative eicosapentaenoic acid (EPA) levels in the exponential phase (25.73% and 28.31%), and highest levels of palmitoleic acid (16:1 n-7) in the stationary phase (46.36% and 43.66%) in the BMB and CCAP strain, respectively. The most striking finding of the VOCs analyses was the relatively high levels of ectocarpene, 6-((1E)-butenyl)-1,4-cycloheptadiene, hormosirene, and desmarestene and structurally related compounds, which were exclusively detected in the BMB strain. Many of the VOCs detected in the CCAP and, in particular, in the BMB strain have been reported as antimicrobial agents. We suggest that the array of pheromones and antimicrobial substances could be part of an allelopathic strategy of the BMB strain, dominated by oval cells, thus reflecting the benthic life stage of this morphological form. These findings show the potential for bioactive metabolites in the BMB strain" |
| Keywords: | Diatoms/*chemistry/metabolism Estuaries Humans Norway Seawater Volatile Organic Compounds/chemistry Water Microbiology Phaeodactylum tricornutum ectocarpene eicosapentaenoic acid (EPA) fatty acids hormosirene two-dimensional gel electrophoresis (2DGE) vol; |
| Notes: | "MedlinePrestegard, Siv Kristin Erga, Svein Rune Steinrucken, Pia Mjos, Svein Are Knutsen, Gjert Rohloff, Jens eng Switzerland 2016/01/06 Mar Drugs. 2015 Dec 30; 14(1):9. doi: 10.3390/md14010009" |
