| Title: | Characterization of Volatile Organic Compounds Emitted from Endophytic Burkholderia cenocepacia ETR-B22 by SPME-GC-MS and Their Inhibitory Activity against Various Plant Fungal Pathogens |
| Author(s): | Chen JH; Xiang W; Cao KX; Lu X; Yao SC; Hung D; Huang RS; Li LB; |
| Address: | "College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, Guangxi, China" |
| DOI: | 10.3390/molecules25173765 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "The use of antagonistic microorganisms and their volatile organic compounds (VOCs) to control plant fungal pathogens is an eco-friendly and promising substitute for chemical fungicides. In this work, endophytic bacterium ETR-B22, isolated from the root of Sophora tonkinensis Gagnep., was found to exhibit strong antagonistic activity against 12 fungal pathogens found in agriculture. Strain ETR-B22 was identified as Burkholderia cenocepacia based on 16S rRNA and recA sequences. We evaluated the antifungal activity of VOCs emitted by ETR-B22. The VOCs from strain ETR-B22 also showed broad-spectrum antifungal activity against 12 fungal pathogens. The composition of the volatile profiles was analyzed based on headspace solid phase microextraction (HS-SPME) gas chromatography coupled to mass spectrometry (GC-MS). Different extraction strategies for the SPME process significantly affected the extraction efficiency of the VOCs. Thirty-two different VOCs were identified. Among the VOC of ETR-B22, dimethyl trisulfide, indole, methyl anthranilate, methyl salicylate, methyl benzoate, benzyl propionate, benzyl acetate, 3,5-di-tert-butylphenol, allyl benzyl ether and nonanoic acid showed broad-spectrum antifungal activity, and are key inhibitory compounds produced by strain ETR-B22 against various fungal pathogens. Our results suggest that the endophytic strain ETR-B22 and its VOCs have high potential for use as biological controls of plant fungal pathogens" |
| Keywords: | "Antifungal Agents/analysis/isolation & purification/*pharmacology Burkholderia cenocepacia/*drug effects/growth & development Gas Chromatography-Mass Spectrometry/*methods Plant Roots/*microbiology RNA, Ribosomal, 16S/analysis Solid Phase Microextraction/;" |
| Notes: | "MedlineChen, Jian-Hua Xiang, Wei Cao, Ke-Xin Lu, Xuan Yao, Shao-Chang Hung, Ding Huang, Rong-Shao Li, Liang-Bo eng Gui Ke AA18118015/Science and Technology Major Project of Guangxi/ Gui Ke AA17204056/Science and Technology Major Project of Guangxi/ Switzerland 2020/08/23 Molecules. 2020 Aug 19; 25(17):3765. doi: 10.3390/molecules25173765" |
