| Title: | Root isoprene formation alters lateral root development |
| Author(s): | Miloradovic van Doorn M; Merl-Pham J; Ghirardo A; Fink S; Polle A; Schnitzler JP; Rosenkranz M; |
| Address: | "Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum Munchen, Neuherberg, Germany. Research Unit Protein Science, Helmholtz Zentrum Munchen, Neuherberg, Germany. Forest Botany, Albert-Ludwigs-Universitat Freiburg, Freiburg im Breisgau, Germany. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China. Forest Botany and Tree Physiology, University of Gottingen, Gottingen, Germany" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analysing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O(2)(-) in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signalling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signalling network and development processes in root tissue" |
| Keywords: | "Alkyl and Aryl Transferases/genetics/metabolism Arabidopsis/genetics Butadienes/*metabolism Hemiterpenes/*metabolism Plant Cells Plant Proteins/genetics/metabolism Plant Roots/cytology/*growth & development/metabolism Plants, Genetically Modified Populus/;" |
| Notes: | "MedlineMiloradovic van Doorn, Maja Merl-Pham, Juliane Ghirardo, Andrea Fink, Siegfried Polle, Andrea Schnitzler, Jorg-Peter Rosenkranz, Maaria eng Research Support, Non-U.S. Gov't 2020/06/05 Plant Cell Environ. 2020 Sep; 43(9):2207-2223. doi: 10.1111/pce.13814. Epub 2020 Jun 27" |
