| Title: | Four 13-lipoxygenases contribute to rapid jasmonate synthesis in wounded Arabidopsis thaliana leaves: a role for lipoxygenase 6 in responses to long-distance wound signals |
| Author(s): | Chauvin A; Caldelari D; Wolfender JL; Farmer EE; |
| Address: | "Department of Plant Molecular Biology, University of Lausanne, Biophore, 1015, Lausanne, Switzerland. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 30 quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland. Swiss Institute of Bioinformatics, University of Lausanne, Genopode, 1015, Lausanne, Switzerland" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Linking) |
| Abstract: | "Damage-inducible defenses in plants are controlled in part by jasmonates, fatty acid-derived regulators that start to accumulate within 30 s of wounding a leaf. Using liquid chromatography-tandem mass spectrometry, we sought to identify the 13-lipoxygenases (13-LOXs) that initiate wound-induced jasmonate synthesis within a 190-s timeframe in Arabidopsis thaliana in 19 single, double, triple and quadruple mutant combinations derived from the four 13-LOX genes in this plant. All four 13-LOXs were found to contribute to jasmonate synthesis in wounded leaves: among them LOX6 showed a unique behavior. The relative contribution of LOX6 to jasmonate synthesis increased with distance from a leaf tip wound, and LOX6 was the only 13-LOX necessary for the initiation of early jasmonate synthesis in leaves distal to the wounded leaf. Herbivory assays that compared Spodoptera littoralis feeding on the lox2-1 lox3B lox4A lox6A quadruple mutant and the lox2-1 lox3B lox4A triple mutant revealed a role for LOX6 in defense of the shoot apical meristem. Consistent with this, we found that LOX6 promoter activity was strong in the apical region of rosettes. The LOX6 promoter was active in and near developing xylem cells and in expression domains we term subtrichomal mounds" |
| Keywords: | "Animals Arabidopsis/*enzymology/genetics/immunology/parasitology Arabidopsis Proteins/genetics/*metabolism Cyclopentanes/*metabolism Gene Expression Regulation, Plant Herbivory/immunology Isoleucine/analogs & derivatives/metabolism Kinetics Lipoxygenase/g;" |
| Notes: | "MedlineChauvin, Adeline Caldelari, Daniela Wolfender, Jean-Luc Farmer, Edward E eng Research Support, Non-U.S. Gov't England 2012/11/23 New Phytol. 2013 Jan; 197(2):566-575. doi: 10.1111/nph.12029. Epub 2012 Nov 21" |
