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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		The volatile molecular profiles of seven Streptococcus pneumoniae serotypes
Author(s):		Mellors TR; Rees CA; Franchina FA; Burklund A; Patel C; Hathaway LJ; Hill JE;
Address:		"Thayer School of Engineering at Dartmouth, 14 Engineering Drive, Hanover, NH 03755, United States. Electronic address: theodore.r.mellors.th@dartmouth.edu. Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, United States. Thayer School of Engineering at Dartmouth, 14 Engineering Drive, Hanover, NH 03755, United States. Institute for Infectious Diseases, Faculty of Medicine, University of Bern, Bern, Switzerland. Thayer School of Engineering at Dartmouth, 14 Engineering Drive, Hanover, NH 03755, United States; Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, United States"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2018
Volume:		20180829
Issue:
Page Number:		208 - 213
DOI:		10.1016/j.jchromb.2018.08.032
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Print) 1570-0232 (Linking)
Abstract:		"In this study, the volatile molecule profile of Streptococcus pneumoniae serotypes was evaluated using solid phase microextraction (SPME) and two dimensional gas chromatography time-of-flight mass spectrometry (GC?ª+x?ª+GC-TOFMS). Here, seven serotypes (6B, 14, 15, 18C, 19F, 9V, and 23F) were analyzed in an isogenic background. We identified 13 core molecules associated with all seven serotypes, and seven molecules that were differentially produced between serotypes. Serotype 14 was found to have the most distinct volatile profile, and could be discriminated from the other six serotypes in aggregate with an area under the curve (AUC) of 89%. This study suggests that molecules from S. pneumoniae culture headspace show potential for rapid serotype identification"
Keywords:		Area Under Curve Bacterial Typing Techniques/*methods Humans Pneumococcal Infections/microbiology Serotyping Streptococcus pneumoniae/*chemistry/*classification/metabolism Volatile Organic Compounds/*analysis/chemistry/metabolism;
Notes:		"MedlineMellors, Theodore R Rees, Christiaan A Franchina, Flavio A Burklund, Alison Patel, Chaya Hathaway, Lucy J Hill, Jane E eng T32 LM012204/LM/NLM NIH HHS/ Netherlands 2018/09/05 J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 1; 1096:208-213. doi: 10.1016/j.jchromb.2018.08.032. Epub 2018 Aug 29"