« Previous AbstractExtraction of phenolic pollutants from industrial wastewater using a bulk ionic liquid membrane technique    Next AbstractGenome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae »

Methods Enzymol

Title:		Identification of yeast pheromone pathway modulators by high-throughput agonist response profiling of a yeast gene knockout strain collection
Author(s):		Chasse SA; Dohlman HG;
Address:		"Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill, 27599-7260, USA"
Journal Title:		Methods Enzymol
Year:		2004
Volume:		389
Issue:
Page Number:		399 - 409
DOI:		10.1016/S0076-6879(04)89024-4
ISSN/ISBN:		0076-6879 (Print) 0076-6879 (Linking)
Abstract:		Gene deletion analysis is a powerful tool for resolving the contributions of individual open reading frames to the physiology of cells. Analysis of deletion phenotypes in conjunction with a specific pathway reporter can identify constituents of a physiological pathway and reveal potential effectors that regulate the pathway by quantifying the phenotypic responses of the mutant cells. This article describes a high-throughput method of analyzing a yeast gene deletion library for novel G-protein signaling modulators using a yeast pheromone pathway-specific reporter
Keywords:		"Data Collection Dose-Response Relationship, Drug Drug Evaluation, Preclinical *Gene Deletion *Gene Expression Profiling Gene Library *Genes, Fungal Genes, Reporter Open Reading Frames Pheromones/genetics/*metabolism/pharmacology Receptors, G-Protein-Coupl;"
Notes:		"MedlineChasse, Scott A Dohlman, Henrik G eng Review 2004/08/18 Methods Enzymol. 2004; 389:399-409. doi: 10.1016/S0076-6879(04)89024-4"