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Vet Res

Title:		The protective role of chicken cathelicidin-1 against Streptococcus suis serotype 2 in vitro and in vivo
Author(s):		Lu Y; Xiang F; Xu L; Tian H; Tao Q; Jia K; Yin H; Ye C; Fang R; Peng L;
Address:		"Joint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing, 400715, China. WestChina-Frontier PharmaTech Co., Ltd, Chengdu, China. Joint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing, 400715, China. rdfang@swu.edu.cn. Chongqing Key Laboratory of Herbivore Science, Chongqing, 400715, China. rdfang@swu.edu.cn. Joint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing, 400715, China. penglianci@swu.edu.cn"
Journal Title:		Vet Res
Year:		2023
Volume:		20230821
Issue:		1
Page Number:		65 -
DOI:		10.1186/s13567-023-01199-1
ISSN/ISBN:		1297-9716 (Electronic) 0928-4249 (Print) 0928-4249 (Linking)
Abstract:		"Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen with the characteristics of high mortality and morbidity, which brings great challenges to prevent and control epidemic disease in the swine industry. Cathelicidins (CATH) are antimicrobial peptides with antimicrobial and immunomodulatory activities. In this study, bactericidal and anti-inflammatory effects of chicken cathelicidin-1 (CATH-1) were investigated in vitro and in vivo against SS2 infection. The results show that CATH-1 exhibited a better bactericidal effect compared to other species' cathelicidins including chickens (CATH-2, -3, and -B1), mice (CRAMP) and pigs (PMAP-36 and PR-39), which rapidly killed bacteria in 20 min by a time-killing curve assay. Furthermore, CATH-1 destroyed the bacterial morphology and affected bacterial ultrastructure as observed under electron microscopy. Moreover, CATH-1 antibacterial activity in vivo shows that CATH-1 increased survival rate of SS2-infected mice by 60% and significantly reduced the bacterial load in the lungs, liver, spleen, blood, and peritoneal lavage as well as the release of SS2-induced inflammatory cytokines including IL-1alpha, IL-1beta, IL-12, and IL-18. Importantly, CATH-1 did not show severe histopathological changes in mice. Further studies on the mechanism of anti-inflammatory activity show that CATH-1 not only reduced the inflammatory response through direct neutralization, but also by regulating the TLR2/4/NF-kappaB/ERK pathway. This study provides a scientific basis for the research and development of antimicrobial peptides as new antimicrobial agents"
Keywords:		Animals Mice Swine *Streptococcus suis Cathelicidins/pharmacology Chickens Serogroup Anti-Bacterial Agents/pharmacology/therapeutic use Antimicrobial Peptides Streptococcus suis anti-inflammatory activity antibacterial activity cathelicidin-1;
Notes:		"MedlineLu, Yi Xiang, Fa Xu, Liuyi Tian, Hongliang Tao, Qi Jia, Kaixiang Yin, Hang Ye, Chao Fang, Rendong Peng, Lianci eng 2021YFD1800800/National Key Research and Development Program of China/ 32102684/National Natural Science Foundation of China/ 32172850/National Natural Science Foundation of China/ cstc2021jcyj-msxm2218/Chongqing Science and Technology Commission/ cstc2021jcyj-msxmX0504/Chongqing Science and Technology Commission/ 20211105/Chongqing Science and Technology Commission/ NCTIP-XD/C17/National Center of Technology Innovation for pigs/ CXQT20004/Foundation for Innovation Research Groups in Chongqing Universities/ England 2023/08/22 Vet Res. 2023 Aug 21; 54(1):65. doi: 10.1186/s13567-023-01199-1"