« Previous AbstractPower and heat measurement by direct calorimetry of individual insect response to allelo- and toxic compounds    Next AbstractVolatile organic compound sensing using a surface-relief D-shaped fiber Bragg grating and a polydimethylsiloxane layer »

J Cell Biol

Title:		Polyester-methacrylate embedments for electron microscopy
Author(s):		Low FN; Clevenger MR;
Address:
Journal Title:		J Cell Biol
Year:		1962
Volume:		12
Issue:		3
Page Number:		615 - 621
DOI:		10.1083/jcb.12.3.615
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"It has been found that tissues fixed for electron microscopy and dehydrated in acetone can be embedded in mixtures of n-butyl methacrylate and polyester resin. Activation with 1 per cent tert-butyl hydroperoxide followed by 12 to 48 hours at 60 degrees C produces blocks that section well with glass knives. The ribbons are cleared of methacrylate by heat (200-250 degrees C for 1 hour) and/or immersion in organic solvents (CCL(4), acetone-ether). After removal of the methacrylate the residual polyester matrix provides thermostable and insoluble support for the tissue. Its insolubility permits staining by immersion of cleared preparations in organic solvents carrying heavy metal compounds in solution. Clearing by heat stabilizes section-grid relationships. The removal of volatile materials by clearing substantially reduces contamination of both specimen and microscope. Tissue fine structure is well preserved in these preparations"
Keywords:		"*Electrons *Histological Techniques *Methacrylates *Microscopy *Microscopy, Electron *Polyesters *Staining and Labeling;"
Notes:		"MedlineLOW, F N CLEVENGER, M R eng 1962/03/01 J Cell Biol. 1962 Mar; 12(3):615-21. doi: 10.1083/jcb.12.3.615"