| Title: | Streptomyces sp. AN090126 as a Biocontrol Agent against Bacterial and Fungal Plant Diseases |
| Author(s): | Le KD; Yu NH; Park AR; Park DJ; Kim CJ; Kim JC; |
| Address: | "Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonam National University, Gwangju 61186, Korea. Institute of New Technology, Academy of Military Science and Technology, 17 Hoangsam, Caugiay, Hanoi 100000, Vietnam. Industrial Bio-Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Korea" |
| DOI: | 10.3390/microorganisms10040791 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "Bacteria and fungi are major phytopathogens which substantially affect global agricultural productivity. In the present study, Streptomyces sp. AN090126, isolated from agricultural suppressive soil in Korea, showed broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi. In the 96-well plate assay, the fermentation filtrate of Streptomyces sp. AN090126 exhibited antimicrobial activity, with a minimum inhibitory concentration (MIC) of 0.63-10% for bacteria and 0.63-3.3% for fungi. The MIC of the partially purified fraction was 20.82-250 microg/mL for bacteria and 15.6-83.33 microg/mL for fungi. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that AN090126 produced various volatile organic compounds (VOCs), including dimethyl sulfide and trimethyl sulfide, which inhibited the growth of pathogenic bacteria and fungi in in vitro VOC assays. In pot experiments, the fermentation broth of Streptomyces sp. AN090126 reduced tomato bacterial wilt caused by Ralstonia solanacearum, red pepper leaf spot caused by Xanthomonas euvesicatoria, and creeping bentgrass dollar spot caused by Sclerotinia homoeocarpa in a dose-dependent manner. Moreover, the secondary metabolites derived from this strain showed a synergistic effect with streptomycin sulfate against streptomycin-resistant Pectobacterium carotovorum subsp. carotovorum, the causative agent of Kimchi cabbage soft rot, in both in vitro and in vivo experiments. Therefore, Streptomyces sp. AN090126 is a potential biocontrol agent in controlling plant diseases caused by pathogenic bacteria and fungi, specifically by the streptomycin-resistant strains" |
| Keywords: | Streptomyces VOCs antimicrobial activity biocontrol agent synergistic effect; |
| Notes: | "PubMed-not-MEDLINELe, Khanh Duy Yu, Nan Hee Park, Ae Ran Park, Dong-Jin Kim, Chang-Jin Kim, Jin-Cheol eng Switzerland 2022/04/24 Microorganisms. 2022 Apr 8; 10(4):791. doi: 10.3390/microorganisms10040791" |
