| Title: | Yeast Associated with Rice Phylloplane and Their Contribution to Control of Rice Sheath Blight Disease |
| Author(s): | Into P; Khunnamwong P; Jindamoragot S; Am-In S; Intanoo W; Limtong S; |
| Address: | "Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology, Development Agency (NSTDA), Pathum Thani 12120, Thailand. Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand. Academy of Science, The Royal Society of Thailand, Bangkok 10300, Thailand" |
| DOI: | 10.3390/microorganisms8030362 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "The phylloplane is an important habitat for yeasts and these yeasts may have antagonistic activities against pathogens and could be used as biocontrol agents. To investigate rice phylloplane yeasts, 282 strains were isolated from 89 rice leaf samples and identified as 15 known yeast species in the phylum Ascomycota and 35 known and two potential new species in the phylum Basidiomycota. The majority of rice phylloplane yeasts belonged to the phylum Basidiomycota. The evaluation of antagonistic activities of 83 yeast strains against rice pathogenic fungi Pyricularia oryzae, Rhizoctonia solani, Fusarium moniliforme, Helminthosporium oryzae and Curvularia lunata revealed that 14 strains inhibited these pathogens. Among the antagonistic strains, Torulaspora indica DMKU-RP31, T. indica DMKU-RP35 and Wickerhamomyces anomalus DMKU-RP25 inhibited all rice pathogens tested, and the production of volatile organic compounds, fungal cell wall degrading enzymes and biofilm were the possible antagonistic mechanisms against all rice pathogens tested in vitro. These yeast strains were evaluated for controlling rice sheath blight caused by R. solani in rice plants in the greenhouse and were found to suppress the disease by 60.0-70.3%, whereas 3% validamycin suppressed by 83.8%. Therefore, they have potential for being developed to be used as biocontrol agents for rice sheath blight" |
| Keywords: | antagonistic yeast biological control phylloplane yeast rice pathogenic fungi rice sheath blight; |
| Notes: | "PubMed-not-MEDLINEInto, Parichat Khunnamwong, Pannida Jindamoragot, Sasitorn Am-In, Somjit Intanoo, Wanwilai Limtong, Savitree eng RTA5480009 and RTA6080004/Thailand Research Fund/ PHD/0025/2556/Royal Golden Jubilee (RGJ) Ph.D. Programme/ Switzerland 2020/03/07 Microorganisms. 2020 Mar 3; 8(3):362. doi: 10.3390/microorganisms8030362" |
