| Title: | Robust Markers of Coffee Consumption Identified Among the Volatile Organic Compounds in Human Urine |
| Author(s): | Mack CI; Egert B; Liberto E; Weinert CH; Bub A; Hoffmann I; Bicchi C; Kulling SE; Cordero C; |
| Address: | "Max Rubner-Institut, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany. Universita degli Studi di Torino, Dipartimento di Scienza e tecnologia del Farmaco, Via Pietro Giuria 9, 10125, Torino, Italy. Max Rubner-Institut, Department of Physiology and Biochemistry of Nutrition, Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany. Max Rubner-Institut, Department of Nutritional Behaviour, Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany" |
| ISSN/ISBN: | 1613-4133 (Electronic) 1613-4125 (Linking) |
| Abstract: | "SCOPE: The human volatilome has gained high interest for the discovery of potential biomarkers of diseases. However, knowledge about the diet as a crucial factor affecting the volatilome is scarce. Therefore, the search for disease biomarkers, as well as the potential use of volatiles as dietary markers is so far limited. The aim of this study is to investigate the association of the diet with the urinary volatilome with the special task to find potential markers of coffee consumption in 24 h urine samples from the Karlsruhe Metabolomics and Nutrition (KarMeN) study. METHODS AND RESULTS: Acidified urine samples are analyzed using an approach combining headspace solid phase microextraction (HS-SPME) sampling with untargeted GCxGC-MS. Overall, 138 reliably occurring volatiles are detected. To account for the unequally concentrated urine samples, results of six different commonly used normalization methods are compared. Statistical analysis evidences six potential markers of coffee consumption, the most promising being 3,4-dimethyl-2,5-furandione. A correlation analysis between the 24 h dietary recall data and the urinary volatilome reveals further promising associations. CONCLUSION: The human urinary volatilome is highly affected by the diet, enabling access to a high level of information about potential diet-related biomarkers. Therefore, it is a very promising source for further investigations on dietary markers" |
| Keywords: | "Adolescent Adult Aged Aged, 80 and over Biomarkers/*urine *Coffee/chemistry Cross-Sectional Studies Eating Female Gas Chromatography-Mass Spectrometry Humans Male Middle Aged Solid Phase Microextraction Volatile Organic Compounds/*urine coffee dietary mar;" |
| Notes: | "MedlineMack, Carina I Egert, Bjorn Liberto, Erica Weinert, Christoph H Bub, Achim Hoffmann, Ingrid Bicchi, Carlo Kulling, Sabine E Cordero, Chiara eng German Federal Ministry of Food and Agriculture/International 2814ERA03E/Bundesministerium fur Ernahrung und Landwirtschaft/International Research Support, Non-U.S. Gov't Germany 2019/03/01 Mol Nutr Food Res. 2019 May; 63(10):e1801060. doi: 10.1002/mnfr.201801060. Epub 2019 Mar 13" |
