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Wei Sheng Wu Xue Bao

Title:		[Phylogenetic diversity characteristics of soil bacteria producing nematode-attracting volatiles and identification of their active compounds]
Author(s):		Hao Y; Mou G; He A; Xi J; Yang F; Mo M;
Address:		"College of Public Health, University of South China, Hengyang 421001, China. haoyuhe@hotmail.com"
Journal Title:		Wei Sheng Wu Xue Bao
Year:		2011
Volume:		51
Issue:		11
Page Number:		1454 - 1460
DOI:
ISSN/ISBN:		0001-6209 (Print) 0001-6209 (Linking)
Abstract:		"OBJECTIVE: This study characterized the phylogenetic diversity of soil bacteria producing nematode-attracting volatiles and their nematode-attracting compounds. Results would enhance our understanding on the interaction between nematodes and soil microorganisms and potentially enhance the biocontrol efficiency when combined the attractants with nemacides. METHODS: Bacteria producing volatiles with functions of nematode-attracting activities were isolated from 187 agricultural soil samples collected in 26 provinces of China with the method of double-Petri dishes. The phylogenetic diversity of these bacteria was characterized by RFLP-16S rRNA gene sequence analysis. The nematode-attracting volatiles of bacteria were detected using the SPME-GC/MS, and volatile compounds with attractive activity were determined by confirming with individual commercial compounds. RESULTS: Among the 3800 bacteria isolated from the 187 soil samples, 196 isolates(5.16% of the total) showed attractive activity (AN)more than 30% to Panagrellus redivivus. Of the 196 isolates, 66 (1.74%) showed AN > or = 70%, 62 isolates (1.63%) showed AN between 50% and 70%, and 68 isolates (1.79%) showed AN less than 50%. Phylogenetic analysis revealed that the 196 bacteria were clustered into 5 groups: Bacilli, Gammaproteobacteria, Alphaproteobacteria, Sphingobacteria and Actinobacteria. But, Bacillus were the dominant, which covered 13 species. And 11 volatiles with nematode-attracting activity were determined, including benzaldehyde, 2-heptanone, benzyl benzoate, ethyl palmitate, (+)-longifolene, benzyl alcohol, p-anisaldehyde, vanatone, ethyl butyrate, isovanilin and d-alaninol. CONCLUSION: Some species of bacteria in agriculture soil can produce volatiles to attract nematodes"
Keywords:		"Animals Bacteria/*genetics/isolation & purification/*metabolism Nematoda/*drug effects Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA/methods Soil Soil Microbiology Volatile Organic Compounds/isolation & purification/*metabolism/*pharmacolo;"
Notes:		"MedlineHao, Yu'e Mou, Guiping He, Aitao Xi, Jiaqin Yang, Faxiang Mo, Minghe chi Research Support, Non-U.S. Gov't China 2012/01/21 Wei Sheng Wu Xue Bao. 2011 Nov 4; 51(11):1454-60"