| Title: | Quantitative Trait Loci Analysis Identifies a Prominent Gene Involved in the Production of Fatty Acid-Derived Flavor Volatiles in Tomato |
| Author(s): | Garbowicz K; Liu Z; Alseekh S; Tieman D; Taylor M; Kuhalskaya A; Ofner I; Zamir D; Klee HJ; Fernie AR; Brotman Y; |
| Address: | "Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany. Horticultural Sciences, Plant Innovation Center, University of Florida, Gainesville, FL, USA. Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany; Center of Plant System Biology and Biotechnology, 4000 Plovdiv, Bulgaria. Department of Life Sciences, Ben-Gurion University of the Negev, Beersheba, Israel. Robert H. Smith Institute of Plant Sciences and Genetics, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 7610001, Israel. Department of Life Sciences, Ben-Gurion University of the Negev, Beersheba, Israel. Electronic address: brotman@mpimp-golm.mpg.de" |
| DOI: | 10.1016/j.molp.2018.06.003 |
| ISSN/ISBN: | 1752-9867 (Electronic) 1674-2052 (Linking) |
| Abstract: | "To gain insight into the genetic regulation of lipid metabolism in tomato, we conducted metabolic trait loci (mQTL) analysis following the lipidomic profiling of fruit pericarp and leaf tissue of the Solanum pennellii introgression lines (IL). To enhance mapping resolution for selected fruit-specific mQTL, we profiled the lipids in a subset of independently derived S. pennellii backcross inbred lines, as well as in a near-isogenic sub-IL population. We identified a putative lecithin:cholesterol acyltransferase that controls the levels of several lipids, and two members of the class III lipase family, LIP1 and LIP2, that were associated with decreased levels of diacylglycerols (DAGs) and triacylglycerols (TAGs). Lipases of this class cleave fatty acids from the glycerol backbone of acylglycerols. The released fatty acids serve as precursors of flavor volatiles. We show that LIP1 expression correlates with fatty acid-derived volatile levels. We further confirm the function of LIP1 in TAG and DAG breakdown and volatile synthesis using transgenic plants. Taken together, our study extensively characterized the genetic architecture of lipophilic compounds in tomato and demonstrated at molecular level that release of free fatty acids from the glycerol backbone can have a major impact on downstream volatile synthesis" |
| Keywords: | "Chromosome Mapping Diglycerides/metabolism Fatty Acids/*metabolism Fruit/genetics/metabolism Gene Expression *Genes, Plant Hybridization, Genetic Lipid Metabolism/genetics Plant Leaves/genetics/metabolism Plant Proteins/genetics/metabolism Plants, Genetic;" |
| Notes: | "MedlineGarbowicz, Karolina Liu, Zhongyuan Alseekh, Saleh Tieman, Denise Taylor, Mark Kuhalskaya, Anastasiya Ofner, Itai Zamir, Dani Klee, Harry J Fernie, Alisdair R Brotman, Yariv eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2018/07/01 Mol Plant. 2018 Sep 10; 11(9):1147-1165. doi: 10.1016/j.molp.2018.06.003. Epub 2018 Jun 28" |
