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Mol Cell

Title:		An SMC-domain protein in fission yeast links telomeres to the meiotic centrosome
Author(s):		Flory MR; Carson AR; Muller EG; Aebersold R;
Address:		"Institute for Systems Biology, Seattle, Washington 98103, USA"
Journal Title:		Mol Cell
Year:		2004
Volume:		16
Issue:		4
Page Number:		619 - 630
DOI:		10.1016/j.molcel.2004.10.027
ISSN/ISBN:		1097-2765 (Print) 1097-2765 (Linking)
Abstract:		"Abnormal centrosomal structures similar to those occurring in human cancers are induced in fission yeast by overexpression of the pericentrin homolog Pcp1p. Analysis of abnormal Pcp1p-containing structures with quantitative mass spectrometry and isotope-coded affinity tags identified a coiled-coil, structural maintenance of chromosomes (SMC) domain protein. This protein, termed Ccq1p (coiled-coil protein quantitatively enriched), localizes with Taz1p to telomeres in normal vegetative cells. Fluorescence resonance energy transfer (FRET) measurements indicate that Ccq1p also interacts with centrosomal Pcp1p in mating pheromone-stimulated cells containing centrosomally clustered telomeres. We provide evidence that the Ccq1p-Pcp1p interaction, while essential for meiosis, is deleterious when forced to occur during vegetative growth. Cells lacking one ccq1 allele exhibit a loss-of-function phenotype including abnormally long cell length, chromosome segregation failure, telomeric shortening, and defective telomeric clustering during meiotic prophase. Our data indicate a mechanism underlying meiotic chromosomal bouquet formation and suggest a recruitment model for supernumerary centrosome toxicity"
Keywords:		"Alleles Cell Nucleus/metabolism Centrosome/*metabolism Chromosomes, Fungal Fluorescence Resonance Energy Transfer Mass Spectrometry Meiosis Microscopy, Fluorescence Models, Biological Models, Molecular Oligonucleotide Array Sequence Analysis Open Reading;"
Notes:		"MedlineFlory, Mark R Carson, Andrew R Muller, Eric G Aebersold, Ruedi eng P41 RR11823/RR/NCRR NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2004/11/18 Mol Cell. 2004 Nov 19; 16(4):619-30. doi: 10.1016/j.molcel.2004.10.027"