| Title: | Rgg-associated SHP signaling peptides mediate cross-talk in Streptococci |
| Author(s): | Fleuchot B; Guillot A; Mezange C; Besset C; Chambellon E; Monnet V; Gardan R; |
| Address: | "INRA, UMR1319 MICALIS, Jouy en Josas, France ; AgroParistech, UMR MICALIS, Jouy en Josas, France. mesabba@umich.edu" |
| DOI: | 10.1371/journal.pone.0066042 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "We described a quorum-sensing mechanism in the streptococci genus involving a short hydrophobic peptide (SHP), which acts as a pheromone, and a transcriptional regulator belonging to the Rgg family. The shp/rgg genes, found in nearly all streptococcal genomes and in several copies in some, have been classified into three groups. We used a genetic approach to evaluate the functionality of the SHP/Rgg quorum-sensing mechanism, encoded by three selected shp/rgg loci, in pathogenic and non-pathogenic streptococci. We characterized the mature form of each SHP pheromone by mass-spectrometry. We produced synthetic peptides corresponding to these mature forms, and used them to study functional complementation and cross-talk between these different SHP/Rgg systems. We demonstrate that a SHP pheromone of one system can influence the activity of a different system. Interestingly, this does not seem to be dependent on the SHP/Rgg group and cross-talk between pathogenic and non-pathogenic streptococci is observed" |
| Keywords: | "Bacterial Proteins/genetics/*metabolism Gene Expression Regulation, Bacterial Peptides/genetics/*metabolism Pheromones/genetics/metabolism Quorum Sensing/genetics/physiology Streptococcus/genetics/*metabolism;" |
| Notes: | "MedlineFleuchot, Betty Guillot, Alain Mezange, Christine Besset, Colette Chambellon, Emilie Monnet, Veronique Gardan, Rozenn eng P30 DK-34933/DK/NIDDK NIH HHS/ R37 DK-041122/DK/NIDDK NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2013/06/19 PLoS One. 2013 Jun 11; 8(6):e66042. doi: 10.1371/journal.pone.0066042. Print 2013" |
