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Mol Plant

Title:		Product variability of the 'cineole cassette' monoterpene synthases of related Nicotiana species
Author(s):		Fahnrich A; Krause K; Piechulla B;
Address:		"University of Rostock, Institute for Biological Sciences, Albert-Einstein-Str. 3, 18059 Rostock, Germany"
Journal Title:		Mol Plant
Year:		2011
Volume:		20110428
Issue:		6
Page Number:		965 - 984
DOI:		10.1093/mp/ssr021
ISSN/ISBN:		1752-9867 (Electronic) 1674-2052 (Linking)
Abstract:		"Nicotiana species of the section Alatae characteristically emit the floral scent compounds of the 'cineole cassette' comprising 1,8-cineole, limonene, myrcene, alpha-pinene, beta-pinene, sabinene, and alpha-terpineol. We successfully isolated genes of Nicotiana alata and Nicotiana langsdorfii that encoded enzymes, which produced the characteristic monoterpenes of this 'cineole cassette' with alpha-terpineol being most abundant in the volatile spectra. The amino acid sequences of both terpineol synthases were 99% identical. The enzymes cluster in a monophyletic branch together with the closely related cineole synthase of Nicotiana suaveolens and monoterpene synthase 1 of Solanum lycopersicum. The cyclization reactions (alpha-terpineol to 1,8-cineole) of the terpineol synthases of N. alata and N. langsdorfii were less efficient compared to the 'cineole cassette' monoterpene synthases of Arabidopsis thaliana, N. suaveolens, Salvia fruticosa, Salvia officinalis, and Citrus unshiu. The terpineol synthases of N. alata and N. langsdorfii were localized in pistils and in the adaxial and abaxial epidermis of the petals. The enzyme activities reached their maxima at the second day after anthesis when flowers were fully opened and the enzyme activity in N. alata was highest at the transition from day to night (diurnal rhythm)"
Keywords:		"Amino Acid Sequence Catalytic Domain Cyclohexanols/*metabolism Eucalyptol Evolution, Molecular Flowers/enzymology/genetics/metabolism Intramolecular Lyases/chemistry/genetics/isolation & purification/*metabolism Models, Molecular Molecular Sequence Data M;"
Notes:		"MedlineFahnrich, Anke Krause, Katrin Piechulla, Birgit eng Research Support, Non-U.S. Gov't England 2011/04/30 Mol Plant. 2011 Nov; 4(6):965-84. doi: 10.1093/mp/ssr021. Epub 2011 Apr 28"