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Science

Title:		Role of a ubiquitin-like modification in polarized morphogenesis
Author(s):		Dittmar GA; Wilkinson CR; Jedrzejewski PT; Finley D;
Address:		"Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA"
Journal Title:		Science
Year:		2002
Volume:		295
Issue:		5564
Page Number:		2442 - 2446
DOI:		10.1126/science.1069989
ISSN/ISBN:		1095-9203 (Electronic) 0036-8075 (Linking)
Abstract:		"Type I ubiquitin-like proteins constitute a family of protein modifiers. Here we report the identification of a posttranslational protein modifier from Saccharomyces cerevisiae, Hub1. Overexpression of Hub1 resulted in enhanced conjugate formation when its carboxyl-terminal residue was deleted, suggesting that mature Hub1 may be produced by proteolytic processing. In vivo targets of Hub1 conjugation included cell polarity factors Sph1 and Hbt1. In the hub1Delta mutant, the subcellular localization of both Hbt1 and Sph1 was disrupted, and cell polarization during the formation of mating projections was defective. Consistent with these polarization defects, the hub1Delta mutant was deficient in mating"
Keywords:		"Amino Acid Sequence Animals Biological Evolution *Cell Polarity Electrophoresis, Gel, Two-Dimensional Gene Deletion Genes, Fungal Humans Ligases/chemistry/genetics/*metabolism Mass Spectrometry Mating Factor *Microfilament Proteins Molecular Sequence Data;"
Notes:		"MedlineDittmar, Gunnar A G Wilkinson, Caroline R M Jedrzejewski, Paul T Finley, Daniel eng GM58223/GM/NIGMS NIH HHS/ GM62663/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2002/03/30 Science. 2002 Mar 29; 295(5564):2442-6. doi: 10.1126/science.1069989"