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Mol Cell Biol

Title:		The yeast alpha 1 and MCM1 proteins bind a single strand of their duplex DNA recognition site
Author(s):		Grayhack EJ;
Address:		"Department of Biology, University of Rochester, New York 14627"
Journal Title:		Mol Cell Biol
Year:		1992
Volume:		12
Issue:		8
Page Number:		3573 - 3582
DOI:		10.1128/mcb.12.8.3573-3582.1992
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"The yeast cell type regulator alpha 1 cooperates with a constitutive factor, MCM1 protein, to recognize the promoter and activate transcription of several alpha-specific genes. I show here that the alpha 1 and MCM1 proteins bind specifically to one of the two strands of their recognition sequence. This single-strand-binding activity shares several characteristics with the duplex-binding properties of these proteins: (i) the MCM1 protein binds alone to single-stranded and duplex sequences of both the alpha-specific (P'Q) and a-specific (P) binding sites; (ii) the alpha 1 protein requires both the MCM1 protein and the Q sequence to bind either single-stranded or duplex DNA; (iii) the alpha 1 protein stimulates binding of the MCM1 protein to both single-stranded and duplex DNAs; and (iv) the affinities of the proteins for single-stranded and duplex DNAs are comparable"
Keywords:		"Base Sequence DNA, Fungal/genetics/isolation & purification/*metabolism DNA-Binding Proteins/*metabolism Fungal Proteins/genetics/isolation & purification/*metabolism *Gene Expression Regulation, Fungal *Genes, Fungal Kinetics Mating Factor Minichromosome;"
Notes:		"MedlineGrayhack, E J eng GM38641/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1992/08/01 Mol Cell Biol. 1992 Aug; 12(8):3573-82. doi: 10.1128/mcb.12.8.3573-3582.1992"