| Title: | "Conjugates of ovarian steroids, including 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (maturation-inducing steroid), accumulate in the urine of a marine teleost (plaice; Pleuronectes platessa)" |
| Address: | "Universidade do Algarve, Faro, Portugal" |
| ISSN/ISBN: | 0022-0795 (Print) 0022-0795 (Linking) |
| Abstract: | "Free and conjugated 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P), 17 alpha,21-dihydroxy-4-pregnene-3,20-dione (11-deoxycortisol) and 3 alpha,17 alpha,21-trihydroxy-5 beta-pregnan-20-one (3 alpha,17,21-P-5 beta) were measured by radioimmunoassay in matching blood plasma and urine samples from plaice (Pleuronectes platessa) females at several ovarian maturity stages: post-vitellogenesis (IV), final oocyte maturation (V), and ovulation (VI). Free steroids were generally low in all samples. Conjugated steroids were up to 2 orders of magnitude higher in urine than in plasma samples. Conjugated 17,20 beta-P was higher in stage V than in stage IV or VI females. Conjugated 11-deoxycortisol was higher in stage IV and V females. Conjugated 3 alpha,17,21-P-5 beta was higher in stage V and VI females. These results support earlier studies which indicated that 17,20 beta-P was the most likely maturation-inducing steroid (MIS) in plaice, and that the urine might be a vehicle for steroid pheromones synthesized by the gonads" |
| Keywords: | Animals Cortodoxone/analogs & derivatives/urine Female Flatfishes/*metabolism Hydroxyprogesterones/*urine Oogenesis Ovulation Radioimmunoassay Time Factors; |
| Notes: | "MedlineCanario, A V Scott, A P eng Research Support, Non-U.S. Gov't England 1989/10/01 J Endocrinol. 1989 Oct; 123(1):R1-4. doi: 10.1677/joe.0.123r001" |
