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Mol Microbiol

Title:		PrgU: a suppressor of sex pheromone toxicity in Enterococcus faecalis
Author(s):		Bhatty M; Camacho MI; Gonzalez-Rivera C; Frank KL; Dale JL; Manias DA; Dunny GM; Christie PJ;
Address:		"Department of Microbiology and Molecular Genetics, McGovern Medical School, 6431 Fannin St, Houston, Texas, 77030, USA. Department of Microbiology and Immunology, University of Minnesota Medical School, Microbiology Research Facility, 689 23rd Ave, S.E, Minneapolis, Minnesota, 55455, USA"
Journal Title:		Mol Microbiol
Year:		2017
Volume:		20161216
Issue:		3
Page Number:		398 - 412
DOI:		10.1111/mmi.13563
ISSN/ISBN:		1365-2958 (Electronic) 0950-382X (Print) 0950-382X (Linking)
Abstract:		"Upon sensing of the peptide pheromone cCF10, Enterococcus faecalis cells carrying pCF10 produce three surface adhesins (PrgA, PrgB or Aggregation Substance, PrgC) and the Prg/Pcf type IV secretion system and, in turn, conjugatively transfer the plasmid at high frequencies to recipient cells. Here, we report that cCF10 induction is highly toxic to cells sustaining a deletion of prgU, a small orf located immediately downstream of prgB on pCF10. Upon pheromone exposure, these cells overproduce the Prg adhesins and display impaired envelope integrity, as evidenced by antibiotic susceptibility, misplaced division septa and cell lysis. Compensatory mutations in regulatory loci controlling expression of pCF10-encoded prg/pcf genes, or constitutive PrgU overproduction, block production of the Prg adhesins and render cells insensitive to pheromone. Cells engineered to overproduce PrgB, even independently of other pCF10-encoded proteins, have severely compromised cell envelopes and strong growth defects. PrgU has an RNA-binding fold, and prgB-prgU gene pairs are widely distributed among E. faecalis isolates and other enterococcal and staphylococcal species. Together, our findings support a model in which PrgU proteins represent a novel class of RNA-binding regulators that act to mitigate toxicity accompanying overproduction of PrgB-like adhesins in E. faecalis and other clinically-important Gram-positive species"
Keywords:		"Amino Acid Sequence/genetics Bacterial Proteins/genetics/metabolism Cell Adhesion Molecules/genetics/metabolism Conjugation, Genetic/genetics DNA, Bacterial/metabolism Enterococcus Enterococcus faecalis/*genetics/*metabolism Gene Expression Regulation, Ba;"
Notes:		"MedlineBhatty, Minny Camacho, Martha I Gonzalez-Rivera, Christian Frank, Kristi L Dale, Jennifer L Manias, Dawn A Dunny, Gary M Christie, Peter J eng R01 GM048746/GM/NIGMS NIH HHS/ R21 AI105454/AI/NIAID NIH HHS/ R35 GM118079/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural England 2016/10/28 Mol Microbiol. 2017 Feb; 103(3):398-412. doi: 10.1111/mmi.13563. Epub 2016 Dec 16"