| Title: | Reproducible breath metabolite changes in children with SARS-CoV-2 infection |
| Author(s): | Berna AZ; Akaho EH; Harris RM; Congdon M; Korn E; Neher S; M'Farrej M; Burns J; John ARO; |
| Address: | "Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA. Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA. Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA" |
| DOI: | 10.1101/2020.12.04.20230755 |
| Abstract: | "SARS-CoV-2 infection is diagnosed through detection of specific viral nucleic acid or antigens from respiratory samples. These techniques are relatively expensive, slow, and susceptible to false-negative results. A rapid non-invasive method to detect infection would be highly advantageous. Compelling evidence from canine biosensors and studies of adults with COVID-19 suggests that infection reproducibly alters human volatile organic compounds (VOCs) profiles. To determine whether pediatric infection is associated with VOC changes, we enrolled SARS-CoV-2-infected and -uninfected children admitted to a major pediatric academic medical center. Breath samples were collected from children and analyzed through state-of-the-art GCxGC-ToFMS. Isolated features included 84 targeted VOCs. Candidate biomarkers that were correlated with infection status were subsequently validated in a second, independent cohort of children. We thus find that six volatile organic compounds are significantly and reproducibly increased in the breath of SARS-CoV-2-infected children. Three aldehydes (octanal, nonanal, and heptanal) drew special attention, as aldehydes are also elevated in the breath of adults with COVID-19. Together, these biomarkers demonstrate high accuracy for distinguishing pediatric SARS-CoV-2 infection and support the ongoing development of novel breath-based diagnostics" |
| Keywords: | Covid-19 SARS-CoV-2 biomarkers breath discovery paediatrics; |
| Notes: | "PubMed-not-MEDLINEBerna, Amalia Z Akaho, Elikplim H Harris, Rebecca M Congdon, Morgan Korn, Emilie Neher, Samuel M'Farrej, Mirna Burns, Julianne John, Audrey R Odom eng R01 AI103280/AI/NIAID NIH HHS/ R21 AI123808/AI/NIAID NIH HHS/ R21 AI130584/AI/NIAID NIH HHS/ Preprint 2020/12/18 medRxiv. 2021 May 7:2020.12.04.20230755. doi: 10.1101/2020.12.04.20230755. Preprint" |
