| Title: | Timing of gene expression in a cell-fate decision system |
| Author(s): | Aymoz D; Sole C; Pierre JJ; Schmitt M; de Nadal E; Posas F; Pelet S; |
| Address: | "Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland. Cell Signaling Research Group, Departament de Ciencies Experimentals i de la Salut, Universitat Pompeu Fabra, Barcelona, Spain. Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland serge.pelet@unil.ch" |
| ISSN/ISBN: | 1744-4292 (Electronic) 1744-4292 (Linking) |
| Abstract: | "During development, morphogens provide extracellular cues allowing cells to select a specific fate by inducing complex transcriptional programs. The mating pathway in budding yeast offers simplified settings to understand this process. Pheromone secreted by the mating partner triggers the activity of a MAPK pathway, which results in the expression of hundreds of genes. Using a dynamic expression reporter, we quantified the kinetics of gene expression in single cells upon exogenous pheromone stimulation and in the physiological context of mating. In both conditions, we observed striking differences in the timing of induction of mating-responsive promoters. Biochemical analyses and generation of synthetic promoter variants demonstrated how the interplay between transcription factor binding and nucleosomes contributes to determine the kinetics of transcription in a simplified cell-fate decision system" |
| Keywords: | "Cell Differentiation/genetics Cell Lineage/genetics Gene Expression Regulation, Fungal/genetics Genes, Mating Type, Fungal/*genetics MAP Kinase Signaling System/genetics Pheromones/*genetics Promoter Regions, Genetic Saccharomyces cerevisiae/*genetics Sac;" |
| Notes: | "MedlineAymoz, Delphine Sole, Carme Pierre, Jean-Jerrold Schmitt, Marta de Nadal, Eulalia Posas, Francesc Pelet, Serge eng Research Support, Non-U.S. Gov't England 2018/04/27 Mol Syst Biol. 2018 Apr 25; 14(4):e8024. doi: 10.15252/msb.20178024" |
