| Title: | Involvement of Membrane Progestin Receptor Beta (mPRbeta/Paqr8) in Sex Pheromone Progestin-Induced Expression of Luteinizing Hormone in the Pituitary of Male Chinese Black Sleeper (Bostrychus Sinensis) |
| Author(s): | Zhang YT; Hong WS; Liu DT; Qiu HT; Zhu Y; Chen SX; |
| Address: | "State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Fujian, China. Department of Biology, East Carolina University, Greenville, NC, United States. Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Fujian, China. State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Fujian, China" |
| Journal Title: | Front Endocrinol (Lausanne) |
| ISSN/ISBN: | 1664-2392 (Print) 1664-2392 (Electronic) 1664-2392 (Linking) |
| Abstract: | "Our previous studies showed that 17alpha, 20beta-dihydroxy-4-pregnen-3-one (DHP) acted as a sex pheromone to induce reproductive success in Chinese black sleeper (Bostrychus sinensis), but its functional mechanism remains unclear. In the present study, we cloned the cDNAs of the gonadotropin subunits (cgalpha, fshbeta, and lhbeta), and found that, in exposure to 5 nM DHP, transcript levels of lhbeta significantly increased in the pituitary at 6 h post exposure; plasma 11-KT levels increased at 24 h post exposure in mature male fish. In contrast, DHP exposure failed to increase the transcript levels of lhbeta in the pituitary of immature male fish, suggesting that the responsiveness to DHP depends on reproductive status. Interestingly, expression of progestin and adipoQ receptor 8 (paqr8, also known as mPRbeta) and progesterone receptor membrane component 2 significantly increased in the olfactory rosette of male fish at late meiosis stage following a co-injection of human chorionic gonadotropin (HCG) and luteinizing hormone releasing hormone-A(3) (LHRH-A(3)), while no increases of other progestin receptors were observed. Moreover, Paqr8 protein was localized in the dendritic knobs of the olfactory sensory neurons, which were activated following the in vivo exposure to DHP. The DHP-induced expression of lhbeta in pituitary was not inhibited by RU486, an antagonist of nuclear progesterone receptor. Taken together, our results suggested that sex pheromone DHP increased the expression of lhbeta transcript in the pituitary and plasma 11-KT levels of mature male, important for reproduction; and Paqr8 might be involved in responding to sex pheromone DHP in the olfactory rosette of male B. sinensis" |
| Keywords: | luteinizing hormone membrane progestin receptor olfactory sensory neuron progestin sex pheromone; |
| Notes: | "PubMed-not-MEDLINEZhang, Yu Ting Hong, Wan Shu Liu, Dong Teng Qiu, Heng Tong Zhu, Yong Chen, Shi Xi eng R15 GM100461/GM/NIGMS NIH HHS/ Switzerland 2018/08/04 Front Endocrinol (Lausanne). 2018 Jul 18; 9:397. doi: 10.3389/fendo.2018.00397. eCollection 2018" |
