| Title: | MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation |
| Author(s): | Zhang JJ; Wang XZ; Luong Do H; Chandimali N; Kang TY; Kim N; Ghosh M; Lee SB; Mok YS; Kim SB; Kwon T; Jeong DK; |
| Address: | "Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju, 63243, Republic of Korea. Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, 400715, P. R. China. Department of Chemical and Biological Engineering, Jeju National University, Jeju, 63243, Republic of Korea. Plasma Technology Research Center, National Fusion Research Institute, Gunsan-si, Jeollabuk-Do, 54004, Republic of Korea. Laboratory of Animal Genetic Engineering and Stem Cell Biology, Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju, 63243, Republic of Korea. taehokwonk@gmail.com. Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju, 63243, Republic of Korea. ngejeong@gmail.com. Laboratory of Animal Genetic Engineering and Stem Cell Biology, Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju, 63243, Republic of Korea. ngejeong@gmail.com" |
| DOI: | 10.1038/s41598-018-27123-8 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Non-thermal plasma treatment is an emerging innovative technique with a wide range of biological applications. This study was conducted to investigate the effect of a non-thermal dielectric barrier discharge plasma technique on immature chicken Sertoli cell (SC) viability and the regulatory role of microRNA (miR)-7450. Results showed that plasma treatment increased SC apoptosis in a time- and dose-dependent manner. Plasma-induced SC apoptosis possibly resulted from the excess production of reactive oxygen species via the suppression of antioxidant defense systems and decreased cellular energy metabolism through the inhibition of adenosine triphosphate (ATP) release and respiratory enzyme activity in the mitochondria. In addition, plasma treatment downregulated miR-7450 expression and activated adenosine monophosphate-activated protein kinase alpha (AMPKalpha), which further inhibited mammalian target of rapamycin (mTOR) phosphorylation in SCs. A single-stranded synthetic miR-7450 antagomir disrupted mitochondrial membrane potential and decreased ATP level and mTOR phosphorylation by targeting the activation of AMPKalpha, which resulted in significant increases in SC lethality. A double-stranded synthetic miR-7450 agomir produced opposite effects on these parameters and ameliorated plasma-mediated apoptotic effects on SCs. Our findings suggest that miR-7450 is involved in the regulation of plasma-induced SC apoptosis through the activation of AMPKalpha and the further inhibition of mTOR signaling pathway" |
| Keywords: | AMP-Activated Protein Kinases/*genetics/metabolism Animals *Apoptosis/drug effects Cell Survival/drug effects Chickens Enzyme Activation/drug effects Equipment Design *Gene Expression Regulation/drug effects Male MicroRNAs/*genetics Mitochondria/drug effe; |
| Notes: | "MedlineZhang, Jiao Jiao Wang, Xian Zhong Luong Do, Huynh Chandimali, Nisansala Kang, Tae Yoon Kim, Nameun Ghosh, Mrinmoy Lee, Sang Baek Mok, Young Sun Kim, Seong Bong Kwon, Taeho Jeong, Dong Kee eng Research Support, Non-U.S. Gov't England 2018/06/10 Sci Rep. 2018 Jun 8; 8(1):8761. doi: 10.1038/s41598-018-27123-8" |
