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Mol Biol Cell

Title:		The mating-specific Galpha interacts with a kinesin-14 and regulates pheromone-induced nuclear migration in budding yeast
Author(s):		Zaichick SV; Metodiev MV; Nelson SA; Durbrovskyi O; Draper E; Cooper JA; Stone DE;
Address:		"Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA"
Journal Title:		Mol Biol Cell
Year:		2009
Volume:		20090422
Issue:		12
Page Number:		2820 - 2830
DOI:		10.1091/mbc.e09-01-0069
ISSN/ISBN:		1939-4586 (Electronic) 1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"As a budding yeast cell elongates toward its mating partner, cytoplasmic microtubules connect the nucleus to the cell cortex at the growth tip. The Kar3 kinesin-like motor protein is then thought to stimulate plus-end depolymerization of these microtubules, thus drawing the nucleus closer to the site where cell fusion and karyogamy will occur. Here, we show that pheromone stimulates a microtubule-independent interaction between Kar3 and the mating-specific Galpha protein Gpa1 and that Gpa1 affects both microtubule orientation and cortical contact. The membrane localization of Gpa1 was found to polarize early in the mating response, at about the same time that the microtubules begin to attach to the incipient growth site. In the absence of Gpa1, microtubules lose contact with the cortex upon shrinking and Kar3 is improperly localized, suggesting that Gpa1 is a cortical anchor for Kar3. We infer that Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating"
Keywords:		"Actins/metabolism Biological Transport/drug effects Cell Nucleus/drug effects/*metabolism Cell Polarity/drug effects Fluorescence Recovery After Photobleaching GTP-Binding Protein alpha Subunits, Gq-G11/deficiency/*metabolism *Genes, Mating Type, Fungal G;"
Notes:		"MedlineZaichick, Sofia V Metodiev, Metodi V Nelson, Scott A Durbrovskyi, Oleksii Draper, Edward Cooper, John A Stone, David E eng R01 GM047337/GM/NIGMS NIH HHS/ GM-47337/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2009/04/24 Mol Biol Cell. 2009 Jun; 20(12):2820-30. doi: 10.1091/mbc.e09-01-0069. Epub 2009 Apr 22"