Title: | Bacterial Species and Biochemical Characteristic Investigations of Nostoc flagelliforme Concentrates during its Storage |
Author(s): | Yue L; Lv H; Zhen J; Jiang S; Jia S; Shen S; Gao L; Dai Y; |
Address: | "Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China. Department of Medicinal Chemistry, Ernest Mario School of Pharmacy, Rutgers University, NJ 08854, USA. Research Center for Modern Analysis Techniques, Tianjin University of Science and Technology, Tianjin 300457, P.R. China" |
ISSN/ISBN: | 1738-8872 (Electronic) 1017-7825 (Linking) |
Abstract: | "Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells" |
Keywords: | Bacteroidetes/genetics/isolation & purification Culture Media Cyanobacteria/genetics/isolation & purification Firmicutes/genetics/isolation & purification Indoles/analysis *Microbial Viability Naphthols/analysis Nostoc/chemistry/*classification/growth & d; |
Notes: | "MedlineYue, Lifang Lv, Hexin Zhen, Jing Jiang, Shengping Jia, Shiru Shen, Shigang Gao, Lu Dai, Yujie eng Korea (South) 2015/12/25 J Microbiol Biotechnol. 2016 Apr 28; 26(4):648-58. doi: 10.4014/jmb.1510.10007" |