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Mol Plant

Title:		"9,10-KODA, an alpha-ketol produced by the tonoplast-localized 9-lipoxygenase ZmLOX5, plays a signaling role in maize defense against insect herbivory"
Author(s):		Yuan P; Borrego E; Park YS; Gorman Z; Huang PC; Tolley J; Christensen SA; Blanford J; Kilaru A; Meeley R; Koiwa H; Vidal S; Huffaker A; Schmelz E; Kolomiets MV;
Address:		"Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77840-2132, USA. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77840-2132, USA; Currently at Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623, USA. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77840-2132, USA; Department of Plant Resources, Agriculture and Fisheries Life Science Research Institute, Kongju National University, Yesan, Chungnam 32439, South Korea. Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843, USA. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77840-2132, USA; College of Life Sciences, Brigham Young University, Provo, UT 84602, USA. Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA. Department of Biological Sciences, East Tennessee State University, Johnson City, TN 37659, USA. Formerly at Corteva Agriscience, Johnston, IA 50131, USA. Department of Crop Sciences, Agricultural Entomology, Georg-August-Universitat, 37077 Gottingen, Germany. Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, CA 92037, USA. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77840-2132, USA. Electronic address: Mike.Kolomiets@ag.tamu.edu"
Journal Title:		Mol Plant
Year:		2023
Volume:		20230711
Issue:		8
Page Number:		1283 - 1303
DOI:		10.1016/j.molp.2023.07.003
ISSN/ISBN:		1752-9867 (Electronic) 1674-2052 (Linking)
Abstract:		"13-Lipoxygenases (LOXs) initiate the synthesis of jasmonic acid (JA), the best-understood oxylipin hormone in herbivory defense. However, the roles of 9-LOX-derived oxylipins in insect resistance remain unclear. Here, we report a novel anti-herbivory mechanism mediated by a tonoplast-localized 9-LOX, ZmLOX5, and its linolenic acid-derived product, 9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid (9,10-KODA). Transposon-insertional disruption of ZmLOX5 resulted in the loss of resistance to insect herbivory. lox5 knockout mutants displayed greatly reduced wound-induced accumulation of multiple oxylipins and defense metabolites, including benzoxazinoids, abscisic acid (ABA), and JA-isoleucine (JA-Ile). However, exogenous JA-Ile failed to rescue insect defense in lox5 mutants, while applications of 1 muM 9,10-KODA or the JA precursor, 12-oxo-phytodienoic acid (12-OPDA), restored wild-type resistance levels. Metabolite profiling revealed that exogenous 9,10-KODA primed the plants for increased production of ABA and 12-OPDA, but not JA-Ile. While none of the 9-oxylipins were able to rescue JA-Ile induction, the lox5 mutant accumulated lower wound-induced levels of Ca(2+), suggesting this as a potential explanation for lower wound-induced JA. Seedlings pretreated with 9,10-KODA exhibited rapid or more robust wound-induced defense gene expression. In addition, an artificial diet supplemented with 9,10-KODA arrested fall armyworm larvae growth. Finally, analysis of single and double lox5 and lox10 mutants showed that ZmLOX5 also contributed to insect defense by modulating ZmLOX10-mediated green leaf volatile signaling. Collectively, our study uncovered a previously unknown anti-herbivore defense and hormone-like signaling activity for a major 9-oxylipin alpha-ketol"
Keywords:		Animals *Oxylipins/metabolism *Zea mays/genetics/metabolism Insecta Abscisic Acid Cyclopentanes/metabolism Hormones Lipoxygenases/genetics 12-oxo-phytodienoic acid Spodoptera benzoxazinoids defense priming fall armyworm oxylipins vacuole;
Notes:		"MedlineYuan, Peiguo Borrego, Eli Park, Yong-Soon Gorman, Zachary Huang, Pei-Cheng Tolley, Jordan Christensen, Shawn A Blanford, Jantana Kilaru, Aruna Meeley, Robert Koiwa, Hisashi Vidal, Stefan Huffaker, Alisa Schmelz, Eric Kolomiets, Michael V eng Research Support, U.S. Gov't, Non-P.H.S. England 2023/07/12 Mol Plant. 2023 Aug 7; 16(8):1283-1303. doi: 10.1016/j.molp.2023.07.003. Epub 2023 Jul 11"