| Title: | "Bacterial Degraders of Coexisting Dichloromethane, Benzene, and Toluene, Identified by Stable-Isotope Probing" |
| Author(s): | Yoshikawa M; Zhang M; Kurisu F; Toyota K; |
| Address: | "Geological Survey of Japan, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Higashi, Tsukuba, Ibaraki 305-8567 Japan. ISNI: 0000 0001 2222 3430. GRID: grid.466781.a Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Koganei, Tokyo 184-8588 Japan. GRID: grid.136594.c Research Center for Water Environment Technology, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-8656 Japan. ISNI: 0000 0001 2151 536X. GRID: grid.26999.3d" |
| DOI: | 10.1007/s11270-017-3604-1 |
| ISSN/ISBN: | 0049-6979 (Print) 1573-2932 (Electronic) 0049-6979 (Linking) |
| Abstract: | "Most bioremediation studies on volatile organic compounds (VOCs) have focused on a single contaminant or its derived compounds and degraders have been identified under single contaminant conditions. Bioremediation of multiple contaminants remains a challenging issue. To identify a bacterial consortium that degrades multiple VOCs (dichloromethane (DCM), benzene, and toluene), we applied DNA-stable isotope probing. For individual tests, we combined a (13)C-labeled VOC with other two unlabeled VOCs, and prepared three unlabeled VOCs as a reference. Over 11 days, DNA was periodically extracted from the consortia, and the bacterial community was evaluated by next-generation sequencing of bacterial 16S rRNA gene amplicons. Density gradient fractions of the DNA extracts were amplified by universal bacterial primers for the 16S rRNA gene sequences, and the amplicons were analyzed by terminal restriction fragment length polymorphism (T-RFLP) using restriction enzymes: HhaI and MspI. The T-RFLP fragments were identified by 16S rRNA gene cloning and sequencing. Under all test conditions, the consortia were dominated by Rhodanobacter, Bradyrhizobium/Afipia, Rhizobium, and Hyphomicrobium. DNA derived from Hyphomicrobium and Propioniferax shifted toward heavier fractions under the condition added with (13)C-DCM and (13)C-benzene, respectively, compared with the reference, but no shifts were induced by (13)C-toluene addition. This implies that Hyphomicrobium and Propioniferax were the main DCM and benzene degraders, respectively, under the coexisting condition. The known benzene degrader Pseudomonas sp. was present but not actively involved in the degradation" |
| Keywords: | Aerobic biodegradation Benzene DNA-stable isotope probing (DNA-SIP) Dichloromethane Multiple volatile organic compounds (multiple VOCs); |
| Notes: | "PubMed-not-MEDLINEYoshikawa, Miho Zhang, Ming Kurisu, Futoshi Toyota, Koki eng Netherlands 2017/11/07 Water Air Soil Pollut. 2017; 228(11):418. doi: 10.1007/s11270-017-3604-1. Epub 2017 Oct 23" |
